Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18155 | 5' | -58.5 | NC_004680.1 | + | 21615 | 0.66 | 0.543693 |
Target: 5'- gGGUGacgcuGCAgGCguucucggccgGGGCCGGggGGg -3' miRNA: 3'- -CCACaucu-CGUgCG-----------CCCGGCCuuCCa -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 51644 | 0.67 | 0.523007 |
Target: 5'- cGUGUGGA-CugGCGGcuCCGgGAAGGUc -3' miRNA: 3'- cCACAUCUcGugCGCCc-GGC-CUUCCA- -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 29299 | 0.67 | 0.512778 |
Target: 5'- cGGUGUccucacuguucgAGGGCaACGUaaGGGCCGGGAa-- -3' miRNA: 3'- -CCACA------------UCUCG-UGCG--CCCGGCCUUcca -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 6663 | 0.67 | 0.492574 |
Target: 5'- cGUGUGGGGUA--CGGGCCGGAu--- -3' miRNA: 3'- cCACAUCUCGUgcGCCCGGCCUucca -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 840 | 0.67 | 0.48261 |
Target: 5'- cGGUGUGGAGCugcaccCGgaGGcGCCGGuucGGUg -3' miRNA: 3'- -CCACAUCUCGu-----GCg-CC-CGGCCuu-CCA- -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 49448 | 0.68 | 0.472744 |
Target: 5'- -uUGUGGAGCGCGCGaaagcagcaCUGGAAGGc -3' miRNA: 3'- ccACAUCUCGUGCGCcc-------GGCCUUCCa -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 42745 | 0.68 | 0.428745 |
Target: 5'- cGUGUAggaggcgccgucgucGAGCAgGCGGGCCGccuGGGc -3' miRNA: 3'- cCACAU---------------CUCGUgCGCCCGGCcu-UCCa -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 55617 | 0.69 | 0.406768 |
Target: 5'- gGGUGUGGuGCgaauguugauuACGCGGuGCCcGGuuGGUg -3' miRNA: 3'- -CCACAUCuCG-----------UGCGCC-CGG-CCuuCCA- -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 19366 | 0.7 | 0.338852 |
Target: 5'- cGGUgGUGGuGGUGCGCaGGGCUGGGccuuGGGUa -3' miRNA: 3'- -CCA-CAUC-UCGUGCG-CCCGGCCU----UCCA- -5' |
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18155 | 5' | -58.5 | NC_004680.1 | + | 50500 | 1 | 0.002546 |
Target: 5'- gGGUGUAGAGC-CGCGGGCCGGAAGGUc -3' miRNA: 3'- -CCACAUCUCGuGCGCCCGGCCUUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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