Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18158 | 3' | -54.5 | NC_004680.1 | + | 57542 | 0.66 | 0.792135 |
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Target: 5'- aCAUCAgCGgugGUuuGggugGCGGCGCGg- -3' miRNA: 3'- gGUAGUaGCa--CAggCa---CGUCGCGCac -5' |
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| 18158 | 3' | -54.5 | NC_004680.1 | + | 16961 | 0.66 | 0.782349 |
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Target: 5'- aCCAUCAUCGccuuuagcUCCcgcGUGCAGgGCGa- -3' miRNA: 3'- -GGUAGUAGCac------AGG---CACGUCgCGCac -5' |
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| 18158 | 3' | -54.5 | NC_004680.1 | + | 48418 | 0.67 | 0.741775 |
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Target: 5'- -gAUCcgCGUGUCCuGgagGCGGUGuCGUGc -3' miRNA: 3'- ggUAGuaGCACAGG-Ca--CGUCGC-GCAC- -5' |
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| 18158 | 3' | -54.5 | NC_004680.1 | + | 29066 | 0.68 | 0.677859 |
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Target: 5'- gCUGUCA--GUGUCCG-GCGGCGCcgcaGUGg -3' miRNA: 3'- -GGUAGUagCACAGGCaCGUCGCG----CAC- -5' |
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| 18158 | 3' | -54.5 | NC_004680.1 | + | 15597 | 0.68 | 0.645174 |
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Target: 5'- aCCAUCGgcgaCGUGUCCagcaGUGgauuGGUGCGUGa -3' miRNA: 3'- -GGUAGUa---GCACAGG----CACg---UCGCGCAC- -5' |
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| 18158 | 3' | -54.5 | NC_004680.1 | + | 52041 | 1.1 | 0.001329 |
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Target: 5'- uCCAUCAUCGUGUCCGUGCAGCGCGUGu -3' miRNA: 3'- -GGUAGUAGCACAGGCACGUCGCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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