Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18158 | 5' | -52 | NC_004680.1 | + | 52261 | 0.66 | 0.868436 |
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Target: 5'- --gGCGCaGCUCUGCGGCGagggcuUCGCgaaguugCGCg -3' miRNA: 3'- aagCGCG-CGAGAUGUUGU------AGUGa------GUG- -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 1276 | 0.66 | 0.85136 |
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Target: 5'- cUUCG-GCauGUUCUGCAACAUCGuCUCGu -3' miRNA: 3'- -AAGCgCG--CGAGAUGUUGUAGU-GAGUg -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 29045 | 0.67 | 0.79453 |
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Target: 5'- -cCGCGCGUUCgGCGGCGUCGaUC-Ca -3' miRNA: 3'- aaGCGCGCGAGaUGUUGUAGUgAGuG- -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 20322 | 0.69 | 0.720161 |
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Target: 5'- -cCGCGCGgaccggugaUCUGCAACAUCACa--- -3' miRNA: 3'- aaGCGCGCg--------AGAUGUUGUAGUGagug -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 38360 | 0.69 | 0.720161 |
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Target: 5'- -aCGCGCuGCggcuUCUACugcuGgAUCGCUCACg -3' miRNA: 3'- aaGCGCG-CG----AGAUGu---UgUAGUGAGUG- -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 37888 | 0.77 | 0.284984 |
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Target: 5'- cUUgGCaCGCUCaACAACAUCACUCAUg -3' miRNA: 3'- -AAgCGcGCGAGaUGUUGUAGUGAGUG- -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 50185 | 0.89 | 0.047823 |
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Target: 5'- cUUgGCGCGCUCaACAACAUCACUCAUg -3' miRNA: 3'- -AAgCGCGCGAGaUGUUGUAGUGAGUG- -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 49522 | 0.99 | 0.009716 |
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Target: 5'- uUUCGCGCGCUCcACAACAUCACUCACu -3' miRNA: 3'- -AAGCGCGCGAGaUGUUGUAGUGAGUG- -5' |
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| 18158 | 5' | -52 | NC_004680.1 | + | 52000 | 1.07 | 0.002414 |
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Target: 5'- uUUCGCGCGCUCUACAACAUCACUCACu -3' miRNA: 3'- -AAGCGCGCGAGAUGUUGUAGUGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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