Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18162 | 3' | -54.8 | NC_004680.1 | + | 27853 | 0.66 | 0.785573 |
Target: 5'- cGGUGGUG-GACGCgcuGGGcgcGAUCGGcGCGa -3' miRNA: 3'- -CCACCGCaCUGUG---UCU---CUAGUC-CGCc -5' |
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18162 | 3' | -54.8 | NC_004680.1 | + | 19566 | 0.66 | 0.789464 |
Target: 5'- cGG-GGgGUGACACGuauuccauugucgcuGAGggCGGcGCGGc -3' miRNA: 3'- -CCaCCgCACUGUGU---------------CUCuaGUC-CGCC- -5' |
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18162 | 3' | -54.8 | NC_004680.1 | + | 17220 | 0.66 | 0.795252 |
Target: 5'- --cGGUGUGGCGCA---AUCGGaGCGGc -3' miRNA: 3'- ccaCCGCACUGUGUcucUAGUC-CGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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