Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18164 | 3' | -51.3 | NC_004680.1 | + | 1642 | 0.66 | 0.893219 |
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Target: 5'- gGAGAGaugCGAcGGCUGUCGUGG--UCa -3' miRNA: 3'- gUUCUCa--GCUaCCGACGGUACUgaAG- -5' |
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| 18164 | 3' | -51.3 | NC_004680.1 | + | 27070 | 0.67 | 0.856248 |
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Target: 5'- gGAGAGUCGuucgaggaacguguUGGCUGCUuGUGGCa-- -3' miRNA: 3'- gUUCUCAGCu-------------ACCGACGG-UACUGaag -5' |
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| 18164 | 3' | -51.3 | NC_004680.1 | + | 30292 | 0.67 | 0.843903 |
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Target: 5'- -cGGGGcgCGAUGGaacgUGCCGUGACa-- -3' miRNA: 3'- guUCUCa-GCUACCg---ACGGUACUGaag -5' |
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| 18164 | 3' | -51.3 | NC_004680.1 | + | 24534 | 0.69 | 0.775742 |
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Target: 5'- uCAGGAGgCGGUGGUgGCgGUGGCggCg -3' miRNA: 3'- -GUUCUCaGCUACCGaCGgUACUGaaG- -5' |
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| 18164 | 3' | -51.3 | NC_004680.1 | + | 38938 | 0.71 | 0.608983 |
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Target: 5'- -uGGAGUCGAUGGC-GUCGUGuauccACUUUg -3' miRNA: 3'- guUCUCAGCUACCGaCGGUAC-----UGAAG- -5' |
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| 18164 | 3' | -51.3 | NC_004680.1 | + | 5447 | 0.73 | 0.508543 |
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Target: 5'- -cGGuGUCGGUGGCcaggGCCGUGGCa-- -3' miRNA: 3'- guUCuCAGCUACCGa---CGGUACUGaag -5' |
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| 18164 | 3' | -51.3 | NC_004680.1 | + | 3739 | 1.11 | 0.001789 |
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Target: 5'- cCAAGAGUCGAUGGCUGCCAUGACUUCg -3' miRNA: 3'- -GUUCUCAGCUACCGACGGUACUGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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