miRNA: 3'- -GGGa---UACuGCGuCGGUAaCUGGCCGU- -5'
miRNA: 3'- -GGGauaCUg--CGU-----------CGGUAACUGGCCGU- -5'
miRNA: 3'- -GGGauaCUGCGUCGGUAA-CUGGCCGU- -5'
miRNA: 3'- gGGAUACUGCGUCgGUAa-----------CUGGCCGu -5'
miRNA: 3'- -GGGAUACUGCGUCGGUAACUGGCCGU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18170 | 3' | -54.7 | NC_004680.1 | + | 34470 | 0.72 | 0.436485 |
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Target: 5'- aCCCgaugGUGuCGC-GUCGUgGACCGGCAc -3' miRNA: 3'- -GGGa---UACuGCGuCGGUAaCUGGCCGU- -5' |
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| 18170 | 3' | -54.7 | NC_004680.1 | + | 9313 | 0.72 | 0.398803 |
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Target: 5'- cCCCgc-GAacaGCAcgcgccaccgcGCCAUUGACCGGCGc -3' miRNA: 3'- -GGGauaCUg--CGU-----------CGGUAACUGGCCGU- -5' |
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| 18170 | 3' | -54.7 | NC_004680.1 | + | 17084 | 0.74 | 0.314214 |
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Target: 5'- uCCC---GGCGCAGCCGUUcGAUUGGCGg -3' miRNA: 3'- -GGGauaCUGCGUCGGUAA-CUGGCCGU- -5' |
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| 18170 | 3' | -54.7 | NC_004680.1 | + | 25777 | 0.74 | 0.305025 |
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Target: 5'- gCCUGUGGCGCAGgCGUguuuguagaugaGGCCGGUg -3' miRNA: 3'- gGGAUACUGCGUCgGUAa-----------CUGGCCGu -5' |
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| 18170 | 3' | -54.7 | NC_004680.1 | + | 7601 | 1.1 | 0.001067 |
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Target: 5'- uCCCUAUGACGCAGCCAUUGACCGGCAc -3' miRNA: 3'- -GGGAUACUGCGUCGGUAACUGGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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