Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18173 | 3' | -56 | NC_004680.1 | + | 6351 | 0.66 | 0.728378 |
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Target: 5'- gGUgUCUGGGAcGGCaGuGUUcacCCGGGACg -3' miRNA: 3'- gCA-AGACCCUuCUGgC-CAA---GGCCCUG- -5' |
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| 18173 | 3' | -56 | NC_004680.1 | + | 47267 | 0.66 | 0.707398 |
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Target: 5'- aGUUCgauuGAGACCGGUUCCGugucGACg -3' miRNA: 3'- gCAAGacccUUCUGGCCAAGGCc---CUG- -5' |
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| 18173 | 3' | -56 | NC_004680.1 | + | 11081 | 0.67 | 0.675421 |
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Target: 5'- ----aUGGGGAGGCCGGUaUCaGGGuCg -3' miRNA: 3'- gcaagACCCUUCUGGCCA-AGgCCCuG- -5' |
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| 18173 | 3' | -56 | NC_004680.1 | + | 51408 | 0.67 | 0.675421 |
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Target: 5'- aGggCcGuGGggGugUGGUgUCCGGGAUg -3' miRNA: 3'- gCaaGaC-CCuuCugGCCA-AGGCCCUG- -5' |
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| 18173 | 3' | -56 | NC_004680.1 | + | 23284 | 0.67 | 0.653888 |
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Target: 5'- gGUgacGuGGAGGuguCCGGUUCgGGGGCg -3' miRNA: 3'- gCAagaC-CCUUCu--GGCCAAGgCCCUG- -5' |
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| 18173 | 3' | -56 | NC_004680.1 | + | 1844 | 0.7 | 0.504953 |
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Target: 5'- ----gUGGGcGGGCCGGUUCCcGGAUc -3' miRNA: 3'- gcaagACCCuUCUGGCCAAGGcCCUG- -5' |
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| 18173 | 3' | -56 | NC_004680.1 | + | 10962 | 0.97 | 0.007806 |
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Target: 5'- cCGUUCUGGGAAGACC-GUUCCGGGACu -3' miRNA: 3'- -GCAAGACCCUUCUGGcCAAGGCCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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