miRNA: 3'- -CGACCACGCC-GAGGGCG--CAACg-GCUU- -5'
miRNA: 3'- cgaCCACGCCGAG-GGCgcaACGGCUU- -5'
miRNA: 3'- -CGACCaCGCCGAgGGCGCAacGGCUu -5'
miRNA: 3'- -CGACC-ACGCCGag----GGCgCAaCGGCUU- -5'
miRNA: 3'- -CGACCACGCCGAGGGCGCAACGGCUU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18176 | 3' | -60.7 | NC_004680.1 | + | 41729 | 0.66 | 0.449168 |
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Target: 5'- cGCUGGUGUGGaCUUCUGCcaGUUGgaUGAAc -3' miRNA: 3'- -CGACCACGCC-GAGGGCG--CAACg-GCUU- -5' |
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| 18176 | 3' | -60.7 | NC_004680.1 | + | 1218 | 0.66 | 0.430329 |
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Target: 5'- --cGGUGCGGCUCaUCGaaaUGCUGAAa -3' miRNA: 3'- cgaCCACGCCGAG-GGCgcaACGGCUU- -5' |
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| 18176 | 3' | -60.7 | NC_004680.1 | + | 35966 | 0.66 | 0.411963 |
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Target: 5'- gGCUGG-GCGGCU-UCGCGU--CCGAu -3' miRNA: 3'- -CGACCaCGCCGAgGGCGCAacGGCUu -5' |
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| 18176 | 3' | -60.7 | NC_004680.1 | + | 47846 | 0.67 | 0.359885 |
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Target: 5'- gGCUGGcUGCGGCgguuggCCGgGUcGCUGAGa -3' miRNA: 3'- -CGACC-ACGCCGag----GGCgCAaCGGCUU- -5' |
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| 18176 | 3' | -60.7 | NC_004680.1 | + | 12813 | 1.07 | 0.000421 |
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Target: 5'- gGCUGGUGCGGCUCCCGCGUUGCCGAAc -3' miRNA: 3'- -CGACCACGCCGAGGGCGCAACGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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