miRNA: 3'- cUGGCCUGGCGGC-GUGGUcuUUACCA- -5'
miRNA: 3'- -CUGGCCugGCGGC-GUGGUCuuUACCA- -5'
miRNA: 3'- -CUGGCCUGGCGGCGUGGUCUUUACCA- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18177 | 5' | -57.4 | NC_004680.1 | + | 12530 | 0.78 | 0.114581 |
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Target: 5'- cAUCGGGCCGCCGcCACCAccGGUGGUg -3' miRNA: 3'- cUGGCCUGGCGGC-GUGGUcuUUACCA- -5' |
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| 18177 | 5' | -57.4 | NC_004680.1 | + | 2859 | 0.79 | 0.099703 |
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Target: 5'- uGACCGGuuCGCCGcCGCCGGugGUGGUg -3' miRNA: 3'- -CUGGCCugGCGGC-GUGGUCuuUACCA- -5' |
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| 18177 | 5' | -57.4 | NC_004680.1 | + | 13163 | 1.08 | 0.000814 |
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Target: 5'- cGACCGGACCGCCGCACCAGAAAUGGUg -3' miRNA: 3'- -CUGGCCUGGCGGCGUGGUCUUUACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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