Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18180 | 5' | -53.5 | NC_004680.1 | + | 30745 | 0.66 | 0.841996 |
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Target: 5'- gCGCAGgGcGACGGcUgGCAGGUCGCg -3' miRNA: 3'- gGUGUCaC-CUGUCcAgCGUCUAGUGa -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 23506 | 0.66 | 0.833121 |
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Target: 5'- gCCACuGUGuuaGACAGGUCuucgGC-GAUCACc -3' miRNA: 3'- -GGUGuCAC---CUGUCCAG----CGuCUAGUGa -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 1389 | 0.66 | 0.833121 |
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Target: 5'- uCgACGGUGGagaaGCGGGUUGCuGcuGUCGCg -3' miRNA: 3'- -GgUGUCACC----UGUCCAGCGuC--UAGUGa -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 54306 | 0.66 | 0.833121 |
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Target: 5'- aCCACGGUGGAguuuCGGucgagugugccGUCGUGGGUgACUu -3' miRNA: 3'- -GGUGUCACCU----GUC-----------CAGCGUCUAgUGA- -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 41521 | 0.66 | 0.824032 |
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Target: 5'- -gGCGGUGacGAUGGGUCGCGu-UCGCUg -3' miRNA: 3'- ggUGUCAC--CUGUCCAGCGUcuAGUGA- -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 18019 | 0.66 | 0.81474 |
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Target: 5'- aCUGCGGUcgGGGCAGGgUGCGGAUgAUUu -3' miRNA: 3'- -GGUGUCA--CCUGUCCaGCGUCUAgUGA- -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 1634 | 0.67 | 0.77574 |
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Target: 5'- gUCGCGGUGGAgAGaugcgacggcuGUCGU-GGUCACUg -3' miRNA: 3'- -GGUGUCACCUgUC-----------CAGCGuCUAGUGA- -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 28823 | 0.69 | 0.680352 |
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Target: 5'- gUC-CGGUGaACAGGUC-CAGGUCGCg -3' miRNA: 3'- -GGuGUCACcUGUCCAGcGUCUAGUGa -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 40829 | 0.74 | 0.370475 |
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Target: 5'- aCCACGGUGuGCGGGUCGCcgacGGuAUCGCc -3' miRNA: 3'- -GGUGUCACcUGUCCAGCG----UC-UAGUGa -5' |
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| 18180 | 5' | -53.5 | NC_004680.1 | + | 15688 | 1.1 | 0.001439 |
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Target: 5'- uCCACAGUGGACAGGUCGCAGAUCACUg -3' miRNA: 3'- -GGUGUCACCUGUCCAGCGUCUAGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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