Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18193 | 3' | -57.3 | NC_004680.1 | + | 24544 | 0.67 | 0.589125 |
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Target: 5'- gUGGUgGCgGUG-GCGGCGCCaaaGUGUu -3' miRNA: 3'- gACUAgUGgCACaCGCCGUGGc--CACA- -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 28300 | 0.67 | 0.578403 |
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Target: 5'- -gGAaCACCG-GUGCcGCACaCGGUGa -3' miRNA: 3'- gaCUaGUGGCaCACGcCGUG-GCCACa -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 19509 | 0.67 | 0.567724 |
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Target: 5'- uUGGUgGCCcggGUGaCGGCgccGCCGGUGg -3' miRNA: 3'- gACUAgUGGca-CAC-GCCG---UGGCCACa -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 15886 | 0.67 | 0.567724 |
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Target: 5'- cCUGA--GCCGgGUGaGGaCACCGGUGUc -3' miRNA: 3'- -GACUagUGGCaCACgCC-GUGGCCACA- -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 1834 | 0.67 | 0.536018 |
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Target: 5'- -cGAUCGCCGcGUG-GGCggGCCGGUu- -3' miRNA: 3'- gaCUAGUGGCaCACgCCG--UGGCCAca -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 34584 | 0.68 | 0.525583 |
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Target: 5'- --cGUCGCCGgggaugGUGCGGUACgGGUu- -3' miRNA: 3'- gacUAGUGGCa-----CACGCCGUGgCCAca -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 39535 | 0.68 | 0.474689 |
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Target: 5'- uUGcAUCGCCGg--GCGGCugCGG-GUg -3' miRNA: 3'- gAC-UAGUGGCacaCGCCGugGCCaCA- -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 43613 | 0.71 | 0.347283 |
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Target: 5'- cCUGGUCgaaCGUG-GCGGCgucgcaACCGGUGUc -3' miRNA: 3'- -GACUAGug-GCACaCGCCG------UGGCCACA- -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 6532 | 0.73 | 0.258399 |
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Target: 5'- gUGcGUCACCGUGUacGCGGUuggaaacgucauaGCCGGUGa -3' miRNA: 3'- gAC-UAGUGGCACA--CGCCG-------------UGGCCACa -5' |
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| 18193 | 3' | -57.3 | NC_004680.1 | + | 28212 | 1.06 | 0.001062 |
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Target: 5'- gCUGAUCACCGUGUGCGGCACCGGUGUu -3' miRNA: 3'- -GACUAGUGGCACACGCCGUGGCCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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