Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18197 | 3' | -58.4 | NC_004680.1 | + | 12581 | 0.66 | 0.56371 |
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Target: 5'- --aCgGCGGCGGUGCGcucggaaUGGCGGu--- -3' miRNA: 3'- uagGgCGCCGCCAUGC-------ACCGCUuaca -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 11071 | 0.66 | 0.52282 |
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Target: 5'- cAUCCUGCugccgGGCGGccUugGUGGCGGc--- -3' miRNA: 3'- -UAGGGCG-----CCGCC--AugCACCGCUuaca -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 55935 | 0.67 | 0.502291 |
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Target: 5'- uUgUCGCGGCGG-GCGUcucgucaaaGGUGAGUGUc -3' miRNA: 3'- uAgGGCGCCGCCaUGCA---------CCGCUUACA- -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 22789 | 0.67 | 0.481125 |
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Target: 5'- uUCCgGUGGCGGUgGCGUucagguaggaggcGGCGAcgGc -3' miRNA: 3'- uAGGgCGCCGCCA-UGCA-------------CCGCUuaCa -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 38290 | 0.68 | 0.452632 |
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Target: 5'- --gCCGCaGCGcGUGCGUGG-GAAUGg -3' miRNA: 3'- uagGGCGcCGC-CAUGCACCgCUUACa -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 42755 | 0.68 | 0.433527 |
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Target: 5'- --aCCGcCGGCaGUGCGcugaaUGGCGAGUGa -3' miRNA: 3'- uagGGC-GCCGcCAUGC-----ACCGCUUACa -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 12225 | 0.68 | 0.432584 |
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Target: 5'- -cCCCGCGaGCGGUuccggugGCGUGGUuccGGUGUc -3' miRNA: 3'- uaGGGCGC-CGCCA-------UGCACCGc--UUACA- -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 24900 | 0.68 | 0.424152 |
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Target: 5'- gGUgCCGcCGGCGGUG-GUGGCGGu--- -3' miRNA: 3'- -UAgGGC-GCCGCCAUgCACCGCUuaca -5' |
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| 18197 | 3' | -58.4 | NC_004680.1 | + | 30492 | 1.06 | 0.0009 |
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Target: 5'- gAUCCCGCGGCGGUACGUGGCGAAUGUg -3' miRNA: 3'- -UAGGGCGCCGCCAUGCACCGCUUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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