Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18199 | 5' | -49.9 | NC_004680.1 | + | 41562 | 0.66 | 0.942042 |
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Target: 5'- cCUUCGGGuacaccAGCGUUGAGggGUgcGCaGACu -3' miRNA: 3'- -GGAGUUC------UCGUAACUCuuCGa-CG-CUG- -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 50095 | 0.66 | 0.941526 |
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Target: 5'- aCCUCAuGAGUgauguuGUUGAGcgcgccaAGGCUGCG-Cu -3' miRNA: 3'- -GGAGUuCUCG------UAACUC-------UUCGACGCuG- -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 23723 | 0.66 | 0.93115 |
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Target: 5'- -aUCGaaGGGGCGcaUGAGggGCUG-GACg -3' miRNA: 3'- ggAGU--UCUCGUa-ACUCuuCGACgCUG- -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 44224 | 0.66 | 0.93115 |
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Target: 5'- aCCUCcgcAGcAGCAgcGAGAAGa-GCGGCa -3' miRNA: 3'- -GGAGu--UC-UCGUaaCUCUUCgaCGCUG- -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 3082 | 0.68 | 0.891301 |
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Target: 5'- gCCgcgcCGGGAGCGUUGuGGGAGUUGCc-- -3' miRNA: 3'- -GGa---GUUCUCGUAAC-UCUUCGACGcug -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 54761 | 0.7 | 0.761663 |
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Target: 5'- -----cGAGCAUUGuuGAGGCUGCGGa -3' miRNA: 3'- ggaguuCUCGUAACu-CUUCGACGCUg -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 10731 | 0.73 | 0.592724 |
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Target: 5'- -aUCAAGGGCGUUGAGuGGCUgGCcACg -3' miRNA: 3'- ggAGUUCUCGUAACUCuUCGA-CGcUG- -5' |
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| 18199 | 5' | -49.9 | NC_004680.1 | + | 32289 | 1.11 | 0.002472 |
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Target: 5'- uCCUCAAGAGCAUUGAGAAGCUGCGACc -3' miRNA: 3'- -GGAGUUCUCGUAACUCUUCGACGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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