Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18201 | 5' | -56.4 | NC_004680.1 | + | 32962 | 0.69 | 0.458854 |
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Target: 5'- gUGCUGCGCAGCAUUacgcagaauugcgcaGUaucgGGGGUcuACCu -3' miRNA: 3'- -ACGACGCGUCGUGG---------------CA----UCCCAcuUGG- -5' |
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| 18201 | 5' | -56.4 | NC_004680.1 | + | 28484 | 0.69 | 0.484906 |
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Target: 5'- aGCcaGgGCAGCGCCGU-GGGUuucGAugCg -3' miRNA: 3'- aCGa-CgCGUCGUGGCAuCCCA---CUugG- -5' |
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| 18201 | 5' | -56.4 | NC_004680.1 | + | 24305 | 0.69 | 0.495113 |
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Target: 5'- cGCcGUGcCGGUGCCGggaAGGGUGGugCc -3' miRNA: 3'- aCGaCGC-GUCGUGGCa--UCCCACUugG- -5' |
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| 18201 | 5' | -56.4 | NC_004680.1 | + | 34150 | 0.68 | 0.515811 |
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Target: 5'- cGCUgcGCGCAGCAaugUCGaGGuGGUGuGCCg -3' miRNA: 3'- aCGA--CGCGUCGU---GGCaUC-CCACuUGG- -5' |
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| 18201 | 5' | -56.4 | NC_004680.1 | + | 26756 | 0.68 | 0.558179 |
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Target: 5'- gGgUGCGUAGCggGCCuucGGGGcgGAACCa -3' miRNA: 3'- aCgACGCGUCG--UGGca-UCCCa-CUUGG- -5' |
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| 18201 | 5' | -56.4 | NC_004680.1 | + | 58778 | 0.66 | 0.663727 |
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Target: 5'- aUGCUGCGCAccuccgccaguGCAUUGguguagcuacggguuGGG-GAGCCg -3' miRNA: 3'- -ACGACGCGU-----------CGUGGCau-------------CCCaCUUGG- -5' |
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| 18201 | 5' | -56.4 | NC_004680.1 | + | 58424 | 0.77 | 0.16156 |
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Target: 5'- gGCUGUGCcgacugcguggaguaGGCGCCGgcaGGUGAGCCa -3' miRNA: 3'- aCGACGCG---------------UCGUGGCaucCCACUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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