Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18201 | 5' | -56.4 | NC_004680.1 | + | 9629 | 0.69 | 0.495113 |
Target: 5'- cUGCUGCuGCGG-GCCGUAacgcuGGUGAGCa -3' miRNA: 3'- -ACGACG-CGUCgUGGCAUc----CCACUUGg -5' |
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18201 | 5' | -56.4 | NC_004680.1 | + | 24305 | 0.69 | 0.495113 |
Target: 5'- cGCcGUGcCGGUGCCGggaAGGGUGGugCc -3' miRNA: 3'- aCGaCGC-GUCGUGGCa--UCCCACUugG- -5' |
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18201 | 5' | -56.4 | NC_004680.1 | + | 28484 | 0.69 | 0.484906 |
Target: 5'- aGCcaGgGCAGCGCCGU-GGGUuucGAugCg -3' miRNA: 3'- aCGa-CgCGUCGUGGCAuCCCA---CUugG- -5' |
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18201 | 5' | -56.4 | NC_004680.1 | + | 32962 | 0.69 | 0.458854 |
Target: 5'- gUGCUGCGCAGCAUUacgcagaauugcgcaGUaucgGGGGUcuACCu -3' miRNA: 3'- -ACGACGCGUCGUGG---------------CA----UCCCAcuUGG- -5' |
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18201 | 5' | -56.4 | NC_004680.1 | + | 53947 | 0.73 | 0.291758 |
Target: 5'- gGCagGUGCAGC-CCGUGGaGGUGAugaGCUg -3' miRNA: 3'- aCGa-CGCGUCGuGGCAUC-CCACU---UGG- -5' |
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18201 | 5' | -56.4 | NC_004680.1 | + | 58424 | 0.77 | 0.16156 |
Target: 5'- gGCUGUGCcgacugcguggaguaGGCGCCGgcaGGUGAGCCa -3' miRNA: 3'- aCGACGCG---------------UCGUGGCaucCCACUUGG- -5' |
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18201 | 5' | -56.4 | NC_004680.1 | + | 32891 | 1.13 | 0.00043 |
Target: 5'- aUGCUGCGCAGCACCGUAGGGUGAACCg -3' miRNA: 3'- -ACGACGCGUCGUGGCAUCCCACUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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