Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18203 | 5' | -53.4 | NC_004680.1 | + | 47224 | 0.66 | 0.817976 |
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Target: 5'- aGUCGUacgGUCCGGGcgguGGCUC-GGGCGg -3' miRNA: 3'- aCAGUAg--CAGGUCUu---UCGGGaCCUGC- -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 21653 | 0.66 | 0.804696 |
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Target: 5'- gUGUCGUCGUCCA---AGCUCacaacgauucuggGGGCGc -3' miRNA: 3'- -ACAGUAGCAGGUcuuUCGGGa------------CCUGC- -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 45426 | 0.67 | 0.798892 |
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Target: 5'- cGUCGUCGUcgagcaacacCCAGuu-GCCCcuguagaGGACGg -3' miRNA: 3'- aCAGUAGCA----------GGUCuuuCGGGa------CCUGC- -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 51701 | 0.67 | 0.758691 |
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Target: 5'- uUGcCGUCGuUCCAGcgcggcucGCCCUGGAUc -3' miRNA: 3'- -ACaGUAGC-AGGUCuuu-----CGGGACCUGc -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 42731 | 0.68 | 0.737755 |
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Target: 5'- cGUCGUCGagCAGGcGGGCcgCCUGGGCc -3' miRNA: 3'- aCAGUAGCagGUCU-UUCG--GGACCUGc -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 45240 | 0.72 | 0.477496 |
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Target: 5'- uUGUCAUCGUUgGGcGGAGUCCUaucGGGCGc -3' miRNA: 3'- -ACAGUAGCAGgUC-UUUCGGGA---CCUGC- -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 19767 | 0.72 | 0.467324 |
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Target: 5'- cGUCGUUccaGUCCGGcggcaaugguGGCCCUGGugGu -3' miRNA: 3'- aCAGUAG---CAGGUCuu--------UCGGGACCugC- -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 33452 | 0.74 | 0.390335 |
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Target: 5'- cG-CAUCGUCCAGGGcuUUCUGGACGa -3' miRNA: 3'- aCaGUAGCAGGUCUUucGGGACCUGC- -5' |
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| 18203 | 5' | -53.4 | NC_004680.1 | + | 33362 | 1.1 | 0.001468 |
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Target: 5'- aUGUCAUCGUCCAGAAAGCCCUGGACGa -3' miRNA: 3'- -ACAGUAGCAGGUCUUUCGGGACCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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