miRNA: 3'- -GGC-----UGaca-UUUaCGCGGGACGGCUCC- -5'
miRNA: 3'- -GGCUGACAUUUACGCgGGa------CG-GCUCC- -5'
miRNA: 3'- -GGCUGACAUUUACGCGGGACGGCUCC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18205 | 3' | -53.9 | NC_004680.1 | + | 34371 | 0.74 | 0.407264 |
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Target: 5'- cCCGccagaACgcaaAAAaGCGCCCUGCCGGGGa -3' miRNA: 3'- -GGC-----UGaca-UUUaCGCGGGACGGCUCC- -5' |
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| 18205 | 3' | -53.9 | NC_004680.1 | + | 51459 | 0.77 | 0.270604 |
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Target: 5'- cUCGGCUGUGGAUGCGgCUggaacugGCcCGAGGa -3' miRNA: 3'- -GGCUGACAUUUACGCgGGa------CG-GCUCC- -5' |
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| 18205 | 3' | -53.9 | NC_004680.1 | + | 34243 | 1.14 | 0.000802 |
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Target: 5'- aCCGACUGUAAAUGCGCCCUGCCGAGGg -3' miRNA: 3'- -GGCUGACAUUUACGCGGGACGGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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