Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1821 | 5' | -57.5 | NC_001347.2 | + | 41274 | 0.67 | 0.883791 |
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Target: 5'- -cCCCGccaacuGGCCGcgcGAACGCGCGUgggCCc -3' miRNA: 3'- aaGGGU------CCGGCu--CUUGCGCGUAa--GGc -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 174649 | 0.67 | 0.869146 |
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Target: 5'- -cUCCGcGGCCGGGAacggugcauuggaACGCGgAUUCCc -3' miRNA: 3'- aaGGGU-CCGGCUCU-------------UGCGCgUAAGGc -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 221952 | 0.68 | 0.847479 |
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Target: 5'- gUUCCaAGcGCCGAGggUagGUGCGUUUCGg -3' miRNA: 3'- -AAGGgUC-CGGCUCuuG--CGCGUAAGGC- -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 67361 | 0.68 | 0.847479 |
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Target: 5'- -gCCCGGGCCaAGAcACGCuGCAcgUCCu -3' miRNA: 3'- aaGGGUCCGGcUCU-UGCG-CGUa-AGGc -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 149581 | 0.69 | 0.783783 |
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Target: 5'- -aCCCAcGGCCGAGAGCcagucgccaccggcuGCGCAgcaacagCCa -3' miRNA: 3'- aaGGGU-CCGGCUCUUG---------------CGCGUaa-----GGc -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 29386 | 0.7 | 0.69519 |
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Target: 5'- -gCCUcaAGGgCGAGAACGCGCccuUUCCu -3' miRNA: 3'- aaGGG--UCCgGCUCUUGCGCGu--AAGGc -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 148610 | 0.75 | 0.453788 |
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Target: 5'- aUCCuCGGGCacgccgagCGAGAGCGCGCAgggUUCCa -3' miRNA: 3'- aAGG-GUCCG--------GCUCUUGCGCGU---AAGGc -5' |
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| 1821 | 5' | -57.5 | NC_001347.2 | + | 8648 | 0.75 | 0.43092 |
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Target: 5'- -cCCCAGGCCGAGuAUGCGCcuagcaugguaauggGUUCUGu -3' miRNA: 3'- aaGGGUCCGGCUCuUGCGCG---------------UAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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