Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18214 | 5' | -51.8 | NC_004680.1 | + | 1834 | 0.66 | 0.922427 |
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Target: 5'- -cGAUCgccGCGUgggCGGG-CCGGUuCCCGGa -3' miRNA: 3'- caCUAGa--UGUA---GCUCaGGCUA-GGGCC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 54482 | 0.66 | 0.921228 |
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Target: 5'- -gGAgCUGCAUgaguucgUGGGUCCacaucggGAUCCCGGu -3' miRNA: 3'- caCUaGAUGUA-------GCUCAGG-------CUAGGGCC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 15988 | 0.67 | 0.873156 |
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Target: 5'- aGUGAUCgugcacGCAUCGAcaCCGGUguccucaCCCGGc -3' miRNA: 3'- -CACUAGa-----UGUAGCUcaGGCUA-------GGGCC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 8820 | 0.68 | 0.849271 |
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Target: 5'- -cGGUCUGCucCGggauguacaGGUCCGGcUCCCGGu -3' miRNA: 3'- caCUAGAUGuaGC---------UCAGGCU-AGGGCC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 19333 | 0.69 | 0.803674 |
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Target: 5'- gGUGAaCUACAUCGAc-CCGGUUgCGGu -3' miRNA: 3'- -CACUaGAUGUAGCUcaGGCUAGgGCC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 28328 | 0.7 | 0.753551 |
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Target: 5'- gGUG-UCgGCGUCGGGgCCGAUCCaccaGGg -3' miRNA: 3'- -CACuAGaUGUAGCUCaGGCUAGGg---CC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 14995 | 0.7 | 0.743105 |
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Target: 5'- uUGAUCgagGCGUCGGGUgCUGA-CCCGc -3' miRNA: 3'- cACUAGa--UGUAGCUCA-GGCUaGGGCc -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 57714 | 0.7 | 0.711132 |
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Target: 5'- uUGGgcggCUGCGgguUCGAGUCgGuguUCCCGGa -3' miRNA: 3'- cACUa---GAUGU---AGCUCAGgCu--AGGGCC- -5' |
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| 18214 | 5' | -51.8 | NC_004680.1 | + | 40428 | 1.1 | 0.002599 |
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Target: 5'- uGUGAUCUACAUCGAGUCCGAUCCCGGc -3' miRNA: 3'- -CACUAGAUGUAGCUCAGGCUAGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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