Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18215 | 5' | -54.5 | NC_004680.1 | + | 26469 | 0.66 | 0.797012 |
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Target: 5'- uCGCCGACaGG-AUCGCUgACCgcaucgccgACAg -3' miRNA: 3'- -GCGGCUG-CCaUAGCGAgUGGaca------UGU- -5' |
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| 18215 | 5' | -54.5 | NC_004680.1 | + | 15737 | 0.66 | 0.777401 |
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Target: 5'- aCGUCGGCuGGUccagguUCGCcCACCUGU-CAg -3' miRNA: 3'- -GCGGCUG-CCAu-----AGCGaGUGGACAuGU- -5' |
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| 18215 | 5' | -54.5 | NC_004680.1 | + | 58068 | 0.67 | 0.715351 |
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Target: 5'- uGCCGAUGGUGUUGgUCACUcGa--- -3' miRNA: 3'- gCGGCUGCCAUAGCgAGUGGaCaugu -5' |
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| 18215 | 5' | -54.5 | NC_004680.1 | + | 26259 | 0.67 | 0.704658 |
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Target: 5'- -cUCGGCGGUGUCGCccUCACCgg-ACu -3' miRNA: 3'- gcGGCUGCCAUAGCG--AGUGGacaUGu -5' |
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| 18215 | 5' | -54.5 | NC_004680.1 | + | 53894 | 0.71 | 0.469171 |
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Target: 5'- uCGCCGGCGuUGUCGC--GCCUGUAg- -3' miRNA: 3'- -GCGGCUGCcAUAGCGagUGGACAUgu -5' |
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| 18215 | 5' | -54.5 | NC_004680.1 | + | 40844 | 0.97 | 0.010641 |
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Target: 5'- uCGCCGACGGUAUCGCcUACCUGUACAa -3' miRNA: 3'- -GCGGCUGCCAUAGCGaGUGGACAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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