Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18219 | 5' | -55 | NC_004680.1 | + | 43498 | 0.65 | 0.807721 |
Target: 5'- gCGACCUagugcguuGACG-ACGCUGCGcaagGAUCGc -3' miRNA: 3'- -GCUGGG--------CUGCuUGCGGCGUa---CUAGCu -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 20011 | 0.65 | 0.807721 |
Target: 5'- cCGAUCCGGUGuugcCGCCGUAUGGgaCGAa -3' miRNA: 3'- -GCUGGGCUGCuu--GCGGCGUACUa-GCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 33993 | 0.65 | 0.807721 |
Target: 5'- uGGCgUGGCGuguuccGCGCUGCugggGUGAUCGGu -3' miRNA: 3'- gCUGgGCUGCu-----UGCGGCG----UACUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 12774 | 0.66 | 0.788744 |
Target: 5'- gCGACCUGGCcgccguacucGAugGCCcGguUGAUCa- -3' miRNA: 3'- -GCUGGGCUG----------CUugCGG-CguACUAGcu -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 4089 | 0.66 | 0.779014 |
Target: 5'- aGACgCGGCGGuagaacuggACGCCGCugcggGUGAaCGAc -3' miRNA: 3'- gCUGgGCUGCU---------UGCGGCG-----UACUaGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 42088 | 0.66 | 0.779014 |
Target: 5'- aGugCCGcCGGGCGCagagguggggCGCAUGAcCGGc -3' miRNA: 3'- gCugGGCuGCUUGCG----------GCGUACUaGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 39865 | 0.66 | 0.778033 |
Target: 5'- -aGCgCCGcagaGAGCGCCGCAUGGagcgccgcacucaUCGAg -3' miRNA: 3'- gcUG-GGCug--CUUGCGGCGUACU-------------AGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 28762 | 0.66 | 0.759125 |
Target: 5'- gGACCCGAUucgcuacuCGCCcaaGUGGUCGAa -3' miRNA: 3'- gCUGGGCUGcuu-----GCGGcg-UACUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 37519 | 0.67 | 0.748987 |
Target: 5'- gCGugCgGAUGAugGUCaGC-UGGUCGAu -3' miRNA: 3'- -GCugGgCUGCUugCGG-CGuACUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 13847 | 0.67 | 0.748987 |
Target: 5'- uGACCUGugG-GCG-CGCAUGGgccgCGAc -3' miRNA: 3'- gCUGGGCugCuUGCgGCGUACUa---GCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 27844 | 0.67 | 0.738734 |
Target: 5'- gCGGCCgGACGGugguggacGCGCUggGCGcGAUCGGc -3' miRNA: 3'- -GCUGGgCUGCU--------UGCGG--CGUaCUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 3734 | 0.67 | 0.738734 |
Target: 5'- gCGGCCCaagaguCGAugGCUGcCAUGAcuUCGGu -3' miRNA: 3'- -GCUGGGcu----GCUugCGGC-GUACU--AGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 47931 | 0.67 | 0.728378 |
Target: 5'- gCGACCCGGCcAAcCGCCGCAgccagcCGGc -3' miRNA: 3'- -GCUGGGCUGcUU-GCGGCGUacua--GCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 33409 | 0.67 | 0.728378 |
Target: 5'- gCGACCagGAauGugGCCGCcuUGGUCGAc -3' miRNA: 3'- -GCUGGg-CUgcUugCGGCGu-ACUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 7090 | 0.67 | 0.728378 |
Target: 5'- cCGACCCGAacuccaCGAgauGCGCCuGCG-GAUCa- -3' miRNA: 3'- -GCUGGGCU------GCU---UGCGG-CGUaCUAGcu -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 55855 | 0.67 | 0.717929 |
Target: 5'- gCGGCuUUGGCGAGCGCCGCuugGAcuaGAa -3' miRNA: 3'- -GCUG-GGCUGCUUGCGGCGua-CUag-CU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 58574 | 0.68 | 0.675421 |
Target: 5'- uGACUgGugGugUGuCUGCGUGGUCGAc -3' miRNA: 3'- gCUGGgCugCuuGC-GGCGUACUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 17340 | 0.69 | 0.610665 |
Target: 5'- -cAUCCGACGAAccCGCCGCA-GGUCc- -3' miRNA: 3'- gcUGGGCUGCUU--GCGGCGUaCUAGcu -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 49965 | 0.69 | 0.610665 |
Target: 5'- aGGCCC-ACGu-CGCCGCcgaGAUCGAc -3' miRNA: 3'- gCUGGGcUGCuuGCGGCGua-CUAGCU- -5' |
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18219 | 5' | -55 | NC_004680.1 | + | 33846 | 0.69 | 0.589125 |
Target: 5'- cCGGCCgaaGACGGACGCUGguUGGcgUGAg -3' miRNA: 3'- -GCUGGg--CUGCUUGCGGCguACUa-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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