Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18221 | 3' | -52.5 | NC_004680.1 | + | 22128 | 0.66 | 0.873425 |
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Target: 5'- gUGUCGCUGUUCCCGacgCCgcgCgcGCAGu- -3' miRNA: 3'- -AUAGUGACAAGGGCa--GGa--Ga-UGUCcc -5' |
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| 18221 | 3' | -52.5 | NC_004680.1 | + | 22817 | 0.68 | 0.762032 |
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Target: 5'- --cCACUGUUCCCGUaagcCUUUUGCAcGGu -3' miRNA: 3'- auaGUGACAAGGGCA----GGAGAUGUcCC- -5' |
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| 18221 | 3' | -52.5 | NC_004680.1 | + | 42931 | 0.7 | 0.653401 |
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Target: 5'- gGUCACgccacgcGUgCuuGUCCUCaACAGGGg -3' miRNA: 3'- aUAGUGa------CAaGggCAGGAGaUGUCCC- -5' |
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| 18221 | 3' | -52.5 | NC_004680.1 | + | 48261 | 0.71 | 0.575419 |
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Target: 5'- gGUCACUGUucgucUCCUGUUgUUgauUGCGGGGg -3' miRNA: 3'- aUAGUGACA-----AGGGCAGgAG---AUGUCCC- -5' |
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| 18221 | 3' | -52.5 | NC_004680.1 | + | 21820 | 0.71 | 0.572108 |
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Target: 5'- -cUCACcGUUCCCGUCCUggucgucguguugaCacACAGGGg -3' miRNA: 3'- auAGUGaCAAGGGCAGGA--------------Ga-UGUCCC- -5' |
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| 18221 | 3' | -52.5 | NC_004680.1 | + | 45314 | 1.1 | 0.001798 |
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Target: 5'- cUAUCACUGUUCCCGUCCUCUACAGGGg -3' miRNA: 3'- -AUAGUGACAAGGGCAGGAGAUGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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