Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18222 | 3' | -55.2 | NC_004680.1 | + | 14312 | 0.66 | 0.783916 |
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Target: 5'- cGGUUGuacGACGGUCCcGGCG-CGGAuGAg -3' miRNA: 3'- -CCAGC---CUGCUAGGaCUGCaGCCU-CUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 7960 | 0.66 | 0.754025 |
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Target: 5'- --cCGGuCGAUUCUGAgGUgaCGGAGAg -3' miRNA: 3'- ccaGCCuGCUAGGACUgCA--GCCUCUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 8781 | 0.66 | 0.743806 |
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Target: 5'- --aCGaGAUGGUCCUGcCGgCGGAGAc -3' miRNA: 3'- ccaGC-CUGCUAGGACuGCaGCCUCUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 24044 | 0.67 | 0.691288 |
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Target: 5'- cGGUC-GAUGGUUCggUGACGUCGGcGAa -3' miRNA: 3'- -CCAGcCUGCUAGG--ACUGCAGCCuCUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 2724 | 0.68 | 0.659027 |
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Target: 5'- aGUCGGauuuuGCGAUUgUGugGUCGGGuGAc -3' miRNA: 3'- cCAGCC-----UGCUAGgACugCAGCCU-CUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 28338 | 0.69 | 0.615718 |
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Target: 5'- cGGcgCGGGCGGUgUcGGCGUCGGGGc- -3' miRNA: 3'- -CCa-GCCUGCUAgGaCUGCAGCCUCua -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 10981 | 0.69 | 0.572625 |
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Target: 5'- cGUCGGGCuGGUCCUGAa-UCGGAa-- -3' miRNA: 3'- cCAGCCUG-CUAGGACUgcAGCCUcua -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 38869 | 0.7 | 0.530281 |
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Target: 5'- cGUUGGAgGGUCCcGGCG-CGGGGGg -3' miRNA: 3'- cCAGCCUgCUAGGaCUGCaGCCUCUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 27095 | 0.71 | 0.459277 |
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Target: 5'- uGUUGGcgGCGcgCCUGuaguCGUCGGAGAg -3' miRNA: 3'- cCAGCC--UGCuaGGACu---GCAGCCUCUa -5' |
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| 18222 | 3' | -55.2 | NC_004680.1 | + | 45488 | 1.08 | 0.00151 |
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Target: 5'- uGGUCGGACGAUCCUGACGUCGGAGAUg -3' miRNA: 3'- -CCAGCCUGCUAGGACUGCAGCCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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