Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18228 | 3' | -58.5 | NC_004680.1 | + | 44546 | 0.66 | 0.584696 |
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Target: 5'- --aGUCCG-ACCCGACGaacgccgcauGAUCGACGc -3' miRNA: 3'- aacCAGGCuUGGGCUGC----------CUGGCUGCa -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 37330 | 0.66 | 0.563626 |
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Target: 5'- -cGGUCUaggGAACUaCGACGGaACCGACc- -3' miRNA: 3'- aaCCAGG---CUUGG-GCUGCC-UGGCUGca -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 3922 | 0.66 | 0.559435 |
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Target: 5'- -cGGUCagcgccguaucgaGAACCUGGCGGgcACCGACc- -3' miRNA: 3'- aaCCAGg------------CUUGGGCUGCC--UGGCUGca -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 39322 | 0.66 | 0.553165 |
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Target: 5'- aUGGUCaaCGAccaACUCGcCGGACCuGACGg -3' miRNA: 3'- aACCAG--GCU---UGGGCuGCCUGG-CUGCa -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 27829 | 0.67 | 0.491854 |
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Target: 5'- gUUGG-CCGcAGCUCGGCGGcCgGACGg -3' miRNA: 3'- -AACCaGGC-UUGGGCUGCCuGgCUGCa -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 29328 | 0.67 | 0.491854 |
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Target: 5'- -aGGgCCGGgaacguGCCCGACaGAUCGACGc -3' miRNA: 3'- aaCCaGGCU------UGGGCUGcCUGGCUGCa -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 23234 | 0.68 | 0.481931 |
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Target: 5'- gUUGGUgCCcgugGAGCCCGACGucCaCGACGUc -3' miRNA: 3'- -AACCA-GG----CUUGGGCUGCcuG-GCUGCA- -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 1662 | 0.68 | 0.452767 |
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Target: 5'- gUGGUCacugaGAuUgCGACGGGCCGGCa- -3' miRNA: 3'- aACCAGg----CUuGgGCUGCCUGGCUGca -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 12461 | 0.69 | 0.415436 |
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Target: 5'- -cGGagCCGGACCCGGCaguGGACCcGCGc -3' miRNA: 3'- aaCCa-GGCUUGGGCUG---CCUGGcUGCa -5' |
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| 18228 | 3' | -58.5 | NC_004680.1 | + | 49727 | 1.05 | 0.001151 |
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Target: 5'- cUUGGUCCGAACCCGACGGACCGACGUg -3' miRNA: 3'- -AACCAGGCUUGGGCUGCCUGGCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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