Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18229 | 3' | -56.8 | NC_004680.1 | + | 23968 | 0.66 | 0.638806 |
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Target: 5'- gCUCGCauCCCg--GUGGGCAguagUGGUu -3' miRNA: 3'- -GAGCGgcGGGacaUACCCGUa---ACCAu -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 34562 | 0.66 | 0.6168 |
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Target: 5'- -gCGCCGCCCaUGuUGUGGGUgccGUcgccggggaUGGUGc -3' miRNA: 3'- gaGCGGCGGG-AC-AUACCCG---UA---------ACCAU- -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 11744 | 0.66 | 0.605813 |
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Target: 5'- gUgGCCGCCCUGaGUGGGguUauaggccgaaaUGGc- -3' miRNA: 3'- gAgCGGCGGGACaUACCCguA-----------ACCau -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 38358 | 0.67 | 0.551388 |
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Target: 5'- uCUCGCCGCUgcccGUGaGGCGUUGGa- -3' miRNA: 3'- -GAGCGGCGGgacaUAC-CCGUAACCau -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 52954 | 0.67 | 0.519434 |
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Target: 5'- uCUCGuCCGCCgcgaggCUGUAccUGGGCAgccuggacugUGGUGg -3' miRNA: 3'- -GAGC-GGCGG------GACAU--ACCCGUa---------ACCAU- -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 36039 | 0.73 | 0.237085 |
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Target: 5'- uUCGCCGCauaaaaaacccccgCCUGUcgGGGCGggGGUu -3' miRNA: 3'- gAGCGGCG--------------GGACAuaCCCGUaaCCAu -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 9807 | 0.75 | 0.173613 |
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Target: 5'- uUCGuCUGCCCUGUcUGGGUAUgagGGUGc -3' miRNA: 3'- gAGC-GGCGGGACAuACCCGUAa--CCAU- -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 36241 | 0.79 | 0.096737 |
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Target: 5'- cCUCGCCGCCCUGc--GGGCAUgGGa- -3' miRNA: 3'- -GAGCGGCGGGACauaCCCGUAaCCau -5' |
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| 18229 | 3' | -56.8 | NC_004680.1 | + | 50348 | 1.07 | 0.000956 |
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Target: 5'- cCUCGCCGCCCUGUAUGGGCAUUGGUAu -3' miRNA: 3'- -GAGCGGCGGGACAUACCCGUAACCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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