Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18233 | 5' | -53.5 | NC_004680.1 | + | 10305 | 0.67 | 0.758541 |
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Target: 5'- aUGCugacCUUGCCGGUGGCgGCgAUUUGc -3' miRNA: 3'- gACGua--GAACGGCUGCCGgCG-UAAAC- -5' |
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| 18233 | 5' | -53.5 | NC_004680.1 | + | 54042 | 0.69 | 0.615936 |
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Target: 5'- -gGCAUCgucUGCgagGACGGCCGCGa--- -3' miRNA: 3'- gaCGUAGa--ACGg--CUGCCGGCGUaaac -5' |
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| 18233 | 5' | -53.5 | NC_004680.1 | + | 59163 | 0.7 | 0.582509 |
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Target: 5'- uUGgGUUgUGCuUGGCGGCCGCAUUg- -3' miRNA: 3'- gACgUAGaACG-GCUGCCGGCGUAAac -5' |
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| 18233 | 5' | -53.5 | NC_004680.1 | + | 9835 | 0.71 | 0.527757 |
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Target: 5'- gCUGCGUCgaagGCUGGCgcgguggcuGGCCGCGcgUUGg -3' miRNA: 3'- -GACGUAGaa--CGGCUG---------CCGGCGUa-AAC- -5' |
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| 18233 | 5' | -53.5 | NC_004680.1 | + | 39535 | 0.72 | 0.464797 |
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Target: 5'- uUGCAUC--GCCgGGCGGCUGCGggUGa -3' miRNA: 3'- gACGUAGaaCGG-CUGCCGGCGUaaAC- -5' |
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| 18233 | 5' | -53.5 | NC_004680.1 | + | 52285 | 0.73 | 0.425066 |
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Target: 5'- cCUGacuCGUCUUGCUGA-GGCCGCGUUc- -3' miRNA: 3'- -GAC---GUAGAACGGCUgCCGGCGUAAac -5' |
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| 18233 | 5' | -53.5 | NC_004680.1 | + | 52604 | 1.09 | 0.001382 |
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Target: 5'- gCUGCAUCUUGCCGACGGCCGCAUUUGg -3' miRNA: 3'- -GACGUAGAACGGCUGCCGGCGUAAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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