Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18235 | 5' | -57.7 | NC_004680.1 | + | 38447 | 0.69 | 0.430418 |
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Target: 5'- cGGCUCcaaCGCCU-CACGGGCaGCGgCg -3' miRNA: 3'- -UCGAGua-GUGGAgGUGCCCGaCGUgG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 57785 | 0.69 | 0.421038 |
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Target: 5'- aGGUUCAg-GCUUUCGCGGGacacgauguugaCUGCGCCg -3' miRNA: 3'- -UCGAGUagUGGAGGUGCCC------------GACGUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 40112 | 0.69 | 0.420106 |
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Target: 5'- gGGC-CGUCGCCUCCuggcgugggggugACGGcccGCUGCgucaGCCg -3' miRNA: 3'- -UCGaGUAGUGGAGG-------------UGCC---CGACG----UGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 7968 | 0.71 | 0.311107 |
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Target: 5'- cGCUCcgCACCaccaUCCucaGGGUUGCAUCc -3' miRNA: 3'- uCGAGuaGUGG----AGGug-CCCGACGUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 5697 | 0.71 | 0.303615 |
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Target: 5'- cAGUUCGUCGCgUUCCAUccgucGGCUGCugCg -3' miRNA: 3'- -UCGAGUAGUG-GAGGUGc----CCGACGugG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 35010 | 0.77 | 0.127951 |
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Target: 5'- gAGUUCGUCAUCaaaCAUGGaGCUGCACCu -3' miRNA: 3'- -UCGAGUAGUGGag-GUGCC-CGACGUGG- -5' |
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| 18235 | 5' | -57.7 | NC_004680.1 | + | 53859 | 1.11 | 0.000414 |
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Target: 5'- cAGCUCAUCACCUCCACGGGCUGCACCu -3' miRNA: 3'- -UCGAGUAGUGGAGGUGCCCGACGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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