Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18240 | 3' | -55.2 | NC_004680.1 | + | 21411 | 0.71 | 0.490288 |
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Target: 5'- -aCGACGACGAucCGGCGAauGGggGCCu- -3' miRNA: 3'- caGCUGCUGCU--GCUGCU--CCaaCGGcu -5' |
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| 18240 | 3' | -55.2 | NC_004680.1 | + | 47245 | 0.72 | 0.450811 |
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Target: 5'- uGUCGACGACGAUGG-GGGGUgaGUCGu -3' miRNA: 3'- -CAGCUGCUGCUGCUgCUCCAa-CGGCu -5' |
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| 18240 | 3' | -55.2 | NC_004680.1 | + | 11127 | 0.73 | 0.385321 |
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Target: 5'- aUCGACGGCGGgagacauCGACGGGGccgggggGCCGGc -3' miRNA: 3'- cAGCUGCUGCU-------GCUGCUCCaa-----CGGCU- -5' |
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| 18240 | 3' | -55.2 | NC_004680.1 | + | 27271 | 0.74 | 0.335904 |
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Target: 5'- -gCGGCGGCGGCGACGcuGccGCCGAg -3' miRNA: 3'- caGCUGCUGCUGCUGCucCaaCGGCU- -5' |
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| 18240 | 3' | -55.2 | NC_004680.1 | + | 23275 | 0.75 | 0.297773 |
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Target: 5'- uUCGACGACgguGACGugGAGGUguCCGGu -3' miRNA: 3'- cAGCUGCUG---CUGCugCUCCAacGGCU- -5' |
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| 18240 | 3' | -55.2 | NC_004680.1 | + | 25707 | 0.84 | 0.082863 |
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Target: 5'- -gCGuCGGCGACGGCGGGGaUGCCGAg -3' miRNA: 3'- caGCuGCUGCUGCUGCUCCaACGGCU- -5' |
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| 18240 | 3' | -55.2 | NC_004680.1 | + | 56341 | 1.09 | 0.001466 |
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Target: 5'- cGUCGACGACGACGACGAGGUUGCCGAc -3' miRNA: 3'- -CAGCUGCUGCUGCUGCUCCAACGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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