Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18241 | 3' | -53.5 | NC_004680.1 | + | 19372 | 0.66 | 0.824433 |
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Target: 5'- uGGuGGuGCGCAgGGCUGGGCcuuggguaUCAACGGu -3' miRNA: 3'- cUCuUC-UGCGU-CUGACCCG--------AGUUGCU- -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 17927 | 0.66 | 0.795755 |
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Target: 5'- cGAGA--ACGCGGAUUGGGUU-GugGAc -3' miRNA: 3'- -CUCUucUGCGUCUGACCCGAgUugCU- -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 23725 | 0.67 | 0.765498 |
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Target: 5'- cGAaGGGGCGCAuGAg-GGGCUgGACGAu -3' miRNA: 3'- -CUcUUCUGCGU-CUgaCCCGAgUUGCU- -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 41543 | 0.67 | 0.755116 |
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Target: 5'- uGAGggGuGCGCAGACUuuccuGGCggUGACGAu -3' miRNA: 3'- -CUCuuC-UGCGUCUGAc----CCGa-GUUGCU- -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 56317 | 0.68 | 0.723241 |
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Target: 5'- uGAGAAcuucGACGCGG-CUGGGgUCGuccaguuugGCGAu -3' miRNA: 3'- -CUCUU----CUGCGUCuGACCCgAGU---------UGCU- -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 8367 | 0.7 | 0.601566 |
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Target: 5'- uGGAugGGugGCccGGGCUGGGCUgAGCa- -3' miRNA: 3'- cUCU--UCugCG--UCUGACCCGAgUUGcu -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 23134 | 0.7 | 0.579393 |
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Target: 5'- cGGGAgcAGACGUcguGGACgucGGGCUCcACGGg -3' miRNA: 3'- -CUCU--UCUGCG---UCUGa--CCCGAGuUGCU- -5' |
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| 18241 | 3' | -53.5 | NC_004680.1 | + | 56582 | 1.08 | 0.001675 |
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Target: 5'- gGAGAAGACGCAGACUGGGCUCAACGAc -3' miRNA: 3'- -CUCUUCUGCGUCUGACCCGAGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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