miRNA: 3'- -UCAGCGGUUUUGUCUGGUGCAGUAGU- -5'
miRNA: 3'- ucAGCGGUUuUGUcUGGUgCAGUAGU- -5'
miRNA: 3'- uCAGC-GGU---UUUGUcUGGUG--CAGUAGU- -5'
miRNA: 3'- -UCaGCGGUUuu------GUCUGGUGCAG--UAGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18241 | 5' | -49.9 | NC_004680.1 | + | 56616 | 1.1 | 0.003427 |
|
Target: 5'- cAGUCGCCAAAACAGACCACGUCAUCAa -3' miRNA: 3'- -UCAGCGGUUUUGUCUGGUGCAGUAGU- -5' |
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| 18241 | 5' | -49.9 | NC_004680.1 | + | 56879 | 0.67 | 0.914138 |
|
Target: 5'- -aUCGCCGAcACAuGCCG-GUCAUCGg -3' miRNA: 3'- ucAGCGGUUuUGUcUGGUgCAGUAGU- -5' |
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| 18241 | 5' | -49.9 | NC_004680.1 | + | 58488 | 0.69 | 0.85277 |
|
Target: 5'- cGUCGaCCAcgcAGACAcACCACcaGUCAUCAc -3' miRNA: 3'- uCAGC-GGU---UUUGUcUGGUG--CAGUAGU- -5' |
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| 18241 | 5' | -49.9 | NC_004680.1 | + | 59361 | 0.69 | 0.85277 |
|
Target: 5'- gGGcCGCCAGcuaacuuuCGGGCCGCGUUcgAUCAg -3' miRNA: 3'- -UCaGCGGUUuu------GUCUGGUGCAG--UAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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