miRNA: 3'- ucAGCGGUU----UUGUCUG--GugCAGUAGU- -5'
miRNA: 3'- uCAGCGGUUUUguCuGGUGC-AgUAGU- -5'
miRNA: 3'- uCAGCGGuUUUGUCuGGUG-CAGUAGU- -5'
miRNA: 3'- -UCAGCGGUUUUGUCUGGUGCAGUAGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18241 | 5' | -49.9 | NC_004680.1 | + | 31695 | 0.72 | 0.710965 |
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Target: 5'- -uUCGCCGAcggcGGCAGACuuUugGUCGUCGu -3' miRNA: 3'- ucAGCGGUU----UUGUCUG--GugCAGUAGU- -5' |
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| 18241 | 5' | -49.9 | NC_004680.1 | + | 5668 | 0.73 | 0.620375 |
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Target: 5'- cGUCGCCGGAAguGuCCGCGcUgGUCAg -3' miRNA: 3'- uCAGCGGUUUUguCuGGUGC-AgUAGU- -5' |
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| 18241 | 5' | -49.9 | NC_004680.1 | + | 19167 | 0.73 | 0.608983 |
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Target: 5'- cGUCGCC-GGAUAGcCCGCcGUCGUCGa -3' miRNA: 3'- uCAGCGGuUUUGUCuGGUG-CAGUAGU- -5' |
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| 18241 | 5' | -49.9 | NC_004680.1 | + | 56616 | 1.1 | 0.003427 |
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Target: 5'- cAGUCGCCAAAACAGACCACGUCAUCAa -3' miRNA: 3'- -UCAGCGGUUUUGUCUGGUGCAGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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