Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18249 | 3' | -55.9 | NC_004681.1 | + | 3614 | 0.66 | 0.815803 |
Target: 5'- -aGUCaGGGAcuUCCCGGcgaggaAGACGUGCg -3' miRNA: 3'- agCAGgCCCUccAGGGUC------UUUGCACG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 28605 | 0.66 | 0.815803 |
Target: 5'- aUGaCCGcGGGGGUgCCCGGGGugaGUGUg -3' miRNA: 3'- aGCaGGC-CCUCCA-GGGUCUUug-CACG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 68113 | 0.66 | 0.806736 |
Target: 5'- cUUG-CCGGGAGG-CCCGaAGGCGUu- -3' miRNA: 3'- -AGCaGGCCCUCCaGGGUcUUUGCAcg -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 49626 | 0.66 | 0.768868 |
Target: 5'- gUCGUCCGGuGAacgagaagGaGUgCCGGGAGCGgGCc -3' miRNA: 3'- -AGCAGGCC-CU--------C-CAgGGUCUUUGCaCG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 24475 | 0.67 | 0.718673 |
Target: 5'- gCG-CCGGGAGaGUUCCAGcgcuACGaGCu -3' miRNA: 3'- aGCaGGCCCUC-CAGGGUCuu--UGCaCG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 15799 | 0.67 | 0.718673 |
Target: 5'- uUCGcCCGGuAGGUgUCCAugaccuccGAGGCGUGCu -3' miRNA: 3'- -AGCaGGCCcUCCA-GGGU--------CUUUGCACG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 58908 | 0.68 | 0.697967 |
Target: 5'- cUGUCCGGGugAGG-CCCAGcaacuccuGGGCGcGCg -3' miRNA: 3'- aGCAGGCCC--UCCaGGGUC--------UUUGCaCG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 49000 | 0.68 | 0.655923 |
Target: 5'- cUUGUaCGGGAcGGUCCCGcGuAGACGUGa -3' miRNA: 3'- -AGCAgGCCCU-CCAGGGU-C-UUUGCACg -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 30921 | 0.69 | 0.592416 |
Target: 5'- -gGUCCGGGcAGGUaggCCCAGGc-CGUGg -3' miRNA: 3'- agCAGGCCC-UCCA---GGGUCUuuGCACg -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 25428 | 0.7 | 0.581892 |
Target: 5'- cCGUCuCGGGAGGggUCGGugGCGUGg -3' miRNA: 3'- aGCAG-GCCCUCCagGGUCuuUGCACg -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 28580 | 0.7 | 0.560969 |
Target: 5'- -aG-CUGGGGGGUCCCAGugguGACGa-- -3' miRNA: 3'- agCaGGCCCUCCAGGGUCu---UUGCacg -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 18933 | 0.7 | 0.557847 |
Target: 5'- cCGUCgCGGGAGcgcagaacggcuuuGUCCCcaagagauucGggGCGUGCa -3' miRNA: 3'- aGCAG-GCCCUC--------------CAGGGu---------CuuUGCACG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 22464 | 0.7 | 0.550583 |
Target: 5'- aCGUCgGGGAGGUCCUcGc--CGUGg -3' miRNA: 3'- aGCAGgCCCUCCAGGGuCuuuGCACg -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 7739 | 0.7 | 0.549548 |
Target: 5'- aCGggCCGGG-GGUCCCccaugagGGAGACGgucugGCc -3' miRNA: 3'- aGCa-GGCCCuCCAGGG-------UCUUUGCa----CG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 59421 | 0.72 | 0.438722 |
Target: 5'- cCGUCCGGGguccuggccacgcaGGaGUCCCcGGAACG-GCa -3' miRNA: 3'- aGCAGGCCC--------------UC-CAGGGuCUUUGCaCG- -5' |
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18249 | 3' | -55.9 | NC_004681.1 | + | 924 | 1.13 | 0.000817 |
Target: 5'- aUCGUCCGGGAGGUCCCAGAAACGUGCg -3' miRNA: 3'- -AGCAGGCCCUCCAGGGUCUUUGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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