Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18249 | 5' | -54.6 | NC_004681.1 | + | 37661 | 0.67 | 0.741384 |
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Target: 5'- -uGCGGGGccUCGCCAGCggccGCgcgUCgCUUCUc -3' miRNA: 3'- cuCGUCCU--AGCGGUCG----UGa--AG-GAAGA- -5' |
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| 18249 | 5' | -54.6 | NC_004681.1 | + | 4168 | 0.67 | 0.741384 |
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Target: 5'- -cGCGGGGUUggcgugggGCCAGCACUccUCgUUCc -3' miRNA: 3'- cuCGUCCUAG--------CGGUCGUGA--AGgAAGa -5' |
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| 18249 | 5' | -54.6 | NC_004681.1 | + | 32219 | 0.67 | 0.709587 |
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Target: 5'- gGAGCAGGccAUCGCagcggCAGCGCggCCUcgugUCUa -3' miRNA: 3'- -CUCGUCC--UAGCG-----GUCGUGaaGGA----AGA- -5' |
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| 18249 | 5' | -54.6 | NC_004681.1 | + | 64756 | 0.68 | 0.677106 |
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Target: 5'- uGGCGGGAUCGuCCGGUAUggCgaUCUg -3' miRNA: 3'- cUCGUCCUAGC-GGUCGUGaaGgaAGA- -5' |
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| 18249 | 5' | -54.6 | NC_004681.1 | + | 52957 | 0.69 | 0.600312 |
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Target: 5'- cGGGCAGGccgAUgGCCAGC-CUUCCg--- -3' miRNA: 3'- -CUCGUCC---UAgCGGUCGuGAAGGaaga -5' |
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| 18249 | 5' | -54.6 | NC_004681.1 | + | 889 | 1.08 | 0.001531 |
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Target: 5'- cGAGCAGGAUCGCCAGCACUUCCUUCUg -3' miRNA: 3'- -CUCGUCCUAGCGGUCGUGAAGGAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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