Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18250 | 3' | -54.3 | NC_004681.1 | + | 26962 | 0.66 | 0.84087 |
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Target: 5'- cGCCAuagGUGGCgGUGgggacc-GGCCCAc -3' miRNA: 3'- -CGGU---UACCGgCACauaagcaCCGGGU- -5' |
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| 18250 | 3' | -54.3 | NC_004681.1 | + | 52298 | 0.66 | 0.815855 |
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Target: 5'- gGCCGuucuUGGCCGUGgauUUCGUGuuuuCUCGg -3' miRNA: 3'- -CGGUu---ACCGGCACau-AAGCACc---GGGU- -5' |
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| 18250 | 3' | -54.3 | NC_004681.1 | + | 30587 | 0.67 | 0.781556 |
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Target: 5'- cGCCAA-GGCgGUucgcugccugacggcGUG--CGUGGCCCAu -3' miRNA: 3'- -CGGUUaCCGgCA---------------CAUaaGCACCGGGU- -5' |
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| 18250 | 3' | -54.3 | NC_004681.1 | + | 28755 | 0.68 | 0.716069 |
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Target: 5'- -aCGGUGucGCCaUGgaGUUCGUGGCCCAg -3' miRNA: 3'- cgGUUAC--CGGcACa-UAAGCACCGGGU- -5' |
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| 18250 | 3' | -54.3 | NC_004681.1 | + | 33469 | 0.8 | 0.172927 |
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Target: 5'- gGCCAGUGGCCGUGgcugAUccUgGUGGCCg- -3' miRNA: 3'- -CGGUUACCGGCACa---UA--AgCACCGGgu -5' |
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| 18250 | 3' | -54.3 | NC_004681.1 | + | 1517 | 1.11 | 0.001323 |
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Target: 5'- cGCCAAUGGCCGUGUAUUCGUGGCCCAu -3' miRNA: 3'- -CGGUUACCGGCACAUAAGCACCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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