Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18251 | 5' | -56.3 | NC_004681.1 | + | 67138 | 0.66 | 0.75832 |
Target: 5'- gCGuGUuGGUGCUGGAgaugguGCCAGUgAGCUu -3' miRNA: 3'- -GCcUAcCCACGACCU------CGGUUAgUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 10298 | 0.66 | 0.727768 |
Target: 5'- aGGuGUGgccGGUGCUccacucGGAGCCGGggaGGCCg -3' miRNA: 3'- gCC-UAC---CCACGA------CCUCGGUUag-UCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 16355 | 0.66 | 0.717388 |
Target: 5'- gCGGcgGUGGGUgGCgacgagGGcGCCAAguggguugccUCGGCCa -3' miRNA: 3'- -GCC--UACCCA-CGa-----CCuCGGUU----------AGUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 16574 | 0.67 | 0.688992 |
Target: 5'- gCGGcgccgaguaguccGUGGGggGCUGGAacgccaccacGCCGAgaccaccaugggucaUCAGCCa -3' miRNA: 3'- -GCC-------------UACCCa-CGACCU----------CGGUU---------------AGUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 35996 | 0.67 | 0.675172 |
Target: 5'- uCGGgcGGGauucGaggaUGGAGCCcgugcaucccAGUCAGCCg -3' miRNA: 3'- -GCCuaCCCa---Cg---ACCUCGG----------UUAGUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 4237 | 0.68 | 0.610874 |
Target: 5'- aGcGAUGGGguUGUUGcGGGCCucagCGGCCu -3' miRNA: 3'- gC-CUACCC--ACGAC-CUCGGuua-GUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 32809 | 0.69 | 0.568231 |
Target: 5'- aGGAcGGcGUGaaGGAGCUGAUCAGgCu -3' miRNA: 3'- gCCUaCC-CACgaCCUCGGUUAGUCgG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 25668 | 0.69 | 0.557676 |
Target: 5'- aGGGUauggccacaGGG-GC-GGGGCCAggcGUCGGCCu -3' miRNA: 3'- gCCUA---------CCCaCGaCCUCGGU---UAGUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 40156 | 0.7 | 0.495755 |
Target: 5'- ---cUGGGUGCUGGcgucGGCCugcggGAUCGGCUu -3' miRNA: 3'- gccuACCCACGACC----UCGG-----UUAGUCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 51751 | 0.7 | 0.489727 |
Target: 5'- gGGGUGGGgaugacgucggaggGCUGGucuuGCCAGgUCcGCCa -3' miRNA: 3'- gCCUACCCa-------------CGACCu---CGGUU-AGuCGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 43785 | 0.72 | 0.382705 |
Target: 5'- gCGGcgGGGUGUguuggaGGGGUUGAUCGGCg -3' miRNA: 3'- -GCCuaCCCACGa-----CCUCGGUUAGUCGg -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 12307 | 0.73 | 0.340923 |
Target: 5'- aCGGAUGaGGUgaccaucguGCUccaGGAGCCGGUCucGCCg -3' miRNA: 3'- -GCCUAC-CCA---------CGA---CCUCGGUUAGu-CGG- -5' |
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18251 | 5' | -56.3 | NC_004681.1 | + | 2094 | 1.02 | 0.003474 |
Target: 5'- cCGGAUGGGUGCUGG-GCCAAUCAGCCc -3' miRNA: 3'- -GCCUACCCACGACCuCGGUUAGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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