Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18253 | 3' | -57.4 | NC_004681.1 | + | 51377 | 0.66 | 0.6626 |
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Target: 5'- gAGGUcggugaugaucuGGUagaCGGUggcgUGGUUGGGGCGGu -3' miRNA: 3'- gUCCG------------CCAg--GUCAa---ACCGAUCCCGUC- -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 4901 | 0.67 | 0.619666 |
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Target: 5'- --cGCGGUCgGGUUUGuC-AGGGCGGa -3' miRNA: 3'- gucCGCCAGgUCAAACcGaUCCCGUC- -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 39151 | 0.67 | 0.591811 |
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Target: 5'- aCAGGUgcuugaccaccuucuGGUCCAGcUUGGgUGGGcGCAc -3' miRNA: 3'- -GUCCG---------------CCAGGUCaAACCgAUCC-CGUc -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 62893 | 0.68 | 0.555735 |
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Target: 5'- uCAGGCGGUUCAccUUGGCguuGGCGa -3' miRNA: 3'- -GUCCGCCAGGUcaAACCGaucCCGUc -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 44173 | 0.68 | 0.555735 |
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Target: 5'- aAGGCGGUuaggCCGGUgaaggUGGUgu-GGCAGg -3' miRNA: 3'- gUCCGCCA----GGUCAa----ACCGaucCCGUC- -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 61851 | 0.68 | 0.534808 |
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Target: 5'- -uGGCGGcCgCGGUcuggUGGCgcgcgAGGGCGGc -3' miRNA: 3'- guCCGCCaG-GUCAa---ACCGa----UCCCGUC- -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 42004 | 0.74 | 0.240725 |
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Target: 5'- aCAGGUGGcguuaUUCAGUUUGGCgcGGGaCAGg -3' miRNA: 3'- -GUCCGCC-----AGGUCAAACCGauCCC-GUC- -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 8892 | 0.74 | 0.22877 |
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Target: 5'- aAGGaagaGGUCCAGcuccUGGCUGGcGGCGGg -3' miRNA: 3'- gUCCg---CCAGGUCaa--ACCGAUC-CCGUC- -5' |
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| 18253 | 3' | -57.4 | NC_004681.1 | + | 4517 | 1.08 | 0.0009 |
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Target: 5'- uCAGGCGGUCCAGUUUGGCUAGGGCAGa -3' miRNA: 3'- -GUCCGCCAGGUCAAACCGAUCCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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