Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
18254 | 3' | -54.5 | NC_004681.1 | + | 62556 | 0.66 | 0.790276 |
Target: 5'- cGGUGUcauccaggagaUGGAacGcCGGGAGCGCGCCGAg -3' miRNA: 3'- uCCGCG-----------AUUU--C-GUCUUCGCGCGGUUg -5' |
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18254 | 3' | -54.5 | NC_004681.1 | + | 28042 | 0.66 | 0.760063 |
Target: 5'- cGGCGCUGGcgcuGGCGgucGAGGCgGUGgCGGCa -3' miRNA: 3'- uCCGCGAUU----UCGU---CUUCG-CGCgGUUG- -5' |
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18254 | 3' | -54.5 | NC_004681.1 | + | 3092 | 0.66 | 0.794198 |
Target: 5'- cGGCGCcAAGcacgucccGCAGGAcuaccugaucggaucGCGCGcCCAGCg -3' miRNA: 3'- uCCGCGaUUU--------CGUCUU---------------CGCGC-GGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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