miRNA: 3'- -UCCGCGaUUUCGu-CU----------UCGCGCGGuUG- -5'
miRNA: 3'- ucCGCgAUUUCGUCuu---CGCG------CGGUUG- -5'
miRNA: 3'- -UCCGCgauuUCGUCUU---------CGCGC-GGUUG- -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18254 | 3' | -54.5 | NC_004681.1 | + | 29810 | 0.67 | 0.707224 |
|
Target: 5'- cAGGCGCgGGAGCucGGcauccaacgcGGCGCGCUuGCc -3' miRNA: 3'- -UCCGCGaUUUCGu-CU----------UCGCGCGGuUG- -5' |
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| 18254 | 3' | -54.5 | NC_004681.1 | + | 38967 | 0.68 | 0.685488 |
|
Target: 5'- -cGCGgUGAAGCAGuucauGCGCugggugGCCGACg -3' miRNA: 3'- ucCGCgAUUUCGUCuu---CGCG------CGGUUG- -5' |
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| 18254 | 3' | -54.5 | NC_004681.1 | + | 39889 | 0.66 | 0.809606 |
|
Target: 5'- cAGGCG----GGCGGAGacccccagcGCGCGgCCAGCu -3' miRNA: 3'- -UCCGCgauuUCGUCUU---------CGCGC-GGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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