Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18254 | 5' | -51.9 | NC_004681.1 | + | 18365 | 0.66 | 0.927566 |
Target: 5'- -cGGUGGCGCca--UCgacaUGGCAGGCc -3' miRNA: 3'- caCCACUGCGucuaAGa---ACCGUUCGc -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 44208 | 0.66 | 0.909023 |
Target: 5'- -gGGUGAgGCAGGUgccuuugUGGCGgugcuucucgaaGGCGg -3' miRNA: 3'- caCCACUgCGUCUAaga----ACCGU------------UCGC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 51523 | 0.66 | 0.909023 |
Target: 5'- aUGGUGGCGUAcg-UCgUGGCGuugAGCGc -3' miRNA: 3'- cACCACUGCGUcuaAGaACCGU---UCGC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 13362 | 0.67 | 0.872595 |
Target: 5'- aUGGccugGACGCGGaAUUC--GGCGAGCu -3' miRNA: 3'- cACCa---CUGCGUC-UAAGaaCCGUUCGc -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 30283 | 0.68 | 0.847636 |
Target: 5'- -cGGUGcCGUcaGGGUUCUUGGCGuaGGUc -3' miRNA: 3'- caCCACuGCG--UCUAAGAACCGU--UCGc -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 44153 | 0.68 | 0.838842 |
Target: 5'- gGUGGUGugGCAGGcccCgcagUGGgAGGUGu -3' miRNA: 3'- -CACCACugCGUCUaa-Ga---ACCgUUCGC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 15648 | 0.68 | 0.820596 |
Target: 5'- -cGGUGcCGguGAUcuUCUUGaCGAGCGa -3' miRNA: 3'- caCCACuGCguCUA--AGAACcGUUCGC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 3813 | 0.68 | 0.811163 |
Target: 5'- gGUuGUGGCGCugaugAGAUUCUugccUGGCAAGaCGg -3' miRNA: 3'- -CAcCACUGCG-----UCUAAGA----ACCGUUC-GC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 25051 | 0.68 | 0.811163 |
Target: 5'- -cGGUGAcCGCAGAgggcgGcGCGGGCGg -3' miRNA: 3'- caCCACU-GCGUCUaagaaC-CGUUCGC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 44807 | 0.71 | 0.675186 |
Target: 5'- gGUGGUGACGCg----CUUGGUgcGCGu -3' miRNA: 3'- -CACCACUGCGucuaaGAACCGuuCGC- -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 19111 | 0.72 | 0.597369 |
Target: 5'- -aGGUGuCGCcg--UCUUGGCGAGCa -3' miRNA: 3'- caCCACuGCGucuaAGAACCGUUCGc -5' |
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18254 | 5' | -51.9 | NC_004681.1 | + | 4808 | 1.09 | 0.002798 |
Target: 5'- cGUGGUGACGCAGAUUCUUGGCAAGCGu -3' miRNA: 3'- -CACCACUGCGUCUAAGAACCGUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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