Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18255 | 3' | -61.8 | NC_004681.1 | + | 6392 | 0.66 | 0.427147 |
Target: 5'- -cGAccGCGGCAGGCCcauGauCUCgCGGCCCu -3' miRNA: 3'- uaCU--CGCUGUCCGG---CgcGAG-GUCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 6344 | 0.66 | 0.44547 |
Target: 5'- ---cGCGAacu-CCGCGCUgCAGCCCu -3' miRNA: 3'- uacuCGCUguccGGCGCGAgGUCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 5808 | 1.09 | 0.000287 |
Target: 5'- cAUGAGCGACAGGCCGCGCUCCAGCCCg -3' miRNA: 3'- -UACUCGCUGUCCGGCGCGAGGUCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 3023 | 0.68 | 0.327287 |
Target: 5'- -cGcGUGGCGGuGCCGuCGC-CCAGCCa -3' miRNA: 3'- uaCuCGCUGUC-CGGC-GCGaGGUCGGg -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 2543 | 0.67 | 0.400519 |
Target: 5'- aGUGGGCgGACGaugucGUCGCGCUgCCAGUCUu -3' miRNA: 3'- -UACUCG-CUGUc----CGGCGCGA-GGUCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 852 | 0.68 | 0.34267 |
Target: 5'- cAUGGGCGGCAgGGCCGgGUa--GGCCg -3' miRNA: 3'- -UACUCGCUGU-CCGGCgCGaggUCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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