Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18255 | 3' | -61.8 | NC_004681.1 | + | 12948 | 0.66 | 0.423536 |
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Target: 5'- cUGAGCGGCAacgucacgcugaccGGCCugacgucGCGCUcaCCGGCUa -3' miRNA: 3'- uACUCGCUGU--------------CCGG-------CGCGA--GGUCGGg -5' |
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| 18255 | 3' | -61.8 | NC_004681.1 | + | 20042 | 0.66 | 0.439927 |
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Target: 5'- -gGA-CGGguGGCCGCGCUaccucugcuacggacCCGGCaCCu -3' miRNA: 3'- uaCUcGCUguCCGGCGCGA---------------GGUCG-GG- -5' |
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| 18255 | 3' | -61.8 | NC_004681.1 | + | 40616 | 0.66 | 0.44547 |
|
Target: 5'- -aGGGCGACGuugaugguGGCCGCcCUcucCCAGCUa -3' miRNA: 3'- uaCUCGCUGU--------CCGGCGcGA---GGUCGGg -5' |
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| 18255 | 3' | -61.8 | NC_004681.1 | + | 6344 | 0.66 | 0.44547 |
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Target: 5'- ---cGCGAacu-CCGCGCUgCAGCCCu -3' miRNA: 3'- uacuCGCUguccGGCGCGAgGUCGGG- -5' |
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| 18255 | 3' | -61.8 | NC_004681.1 | + | 31391 | 0.66 | 0.464225 |
|
Target: 5'- -aGGGCuACGGGgCGCGCgacggaCuGCCCg -3' miRNA: 3'- uaCUCGcUGUCCgGCGCGag----GuCGGG- -5' |
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| 18255 | 3' | -61.8 | NC_004681.1 | + | 16448 | 0.72 | 0.177262 |
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Target: 5'- gGUGGucuCGGCGuGGUgGCGUUCCAGCCCc -3' miRNA: 3'- -UACUc--GCUGU-CCGgCGCGAGGUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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