Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18255 | 3' | -61.8 | NC_004681.1 | + | 62319 | 0.7 | 0.233975 |
Target: 5'- -cGcGCGcACAGGUgGCGgUaCCAGCCCa -3' miRNA: 3'- uaCuCGC-UGUCCGgCGCgA-GGUCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 16249 | 0.71 | 0.228245 |
Target: 5'- gAUGAGCGACGGGauG-GCcgCCAGCgCCg -3' miRNA: 3'- -UACUCGCUGUCCggCgCGa-GGUCG-GG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 49666 | 0.72 | 0.196319 |
Target: 5'- -cGAGCGugaaGCAGGCuacugCGCGCUCUgcGGCCUc -3' miRNA: 3'- uaCUCGC----UGUCCG-----GCGCGAGG--UCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 16448 | 0.72 | 0.177262 |
Target: 5'- gGUGGucuCGGCGuGGUgGCGUUCCAGCCCc -3' miRNA: 3'- -UACUc--GCUGU-CCGgCGCGAGGUCGGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 53429 | 0.74 | 0.136656 |
Target: 5'- cGUGAGCG-CAGGUacuuguaGUGCUCCAGgaCCCg -3' miRNA: 3'- -UACUCGCuGUCCGg------CGCGAGGUC--GGG- -5' |
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18255 | 3' | -61.8 | NC_004681.1 | + | 5808 | 1.09 | 0.000287 |
Target: 5'- cAUGAGCGACAGGCCGCGCUCCAGCCCg -3' miRNA: 3'- -UACUCGCUGUCCGGCGCGAGGUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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