Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18256 | 5' | -56.8 | NC_004681.1 | + | 17035 | 0.66 | 0.764299 |
Target: 5'- aGCGUCGAgcgcUUCGuuCUUgGCCUUCUGGa -3' miRNA: 3'- -CGCAGCU----GAGCcuGAAgCGGAGGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 14562 | 0.66 | 0.754486 |
Target: 5'- gGCGgaggCGGCgcgCGcGGCgUUCGCCUCCg-- -3' miRNA: 3'- -CGCa---GCUGa--GC-CUG-AAGCGGAGGguc -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 11484 | 0.66 | 0.754486 |
Target: 5'- uGCGcaUCcGCUCguGGACUUCGUCagCCAGg -3' miRNA: 3'- -CGC--AGcUGAG--CCUGAAGCGGagGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 39990 | 0.66 | 0.748542 |
Target: 5'- ---cCGGCUCGGccuugaaccagcgccGCUUgGCCUgCCAGg -3' miRNA: 3'- cgcaGCUGAGCC---------------UGAAgCGGAgGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 29546 | 0.66 | 0.734525 |
Target: 5'- ---cCGGCUCGGGCaUCGCCg-CCAa -3' miRNA: 3'- cgcaGCUGAGCCUGaAGCGGagGGUc -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 34584 | 0.67 | 0.703903 |
Target: 5'- uGUG-CGcCUCGGugUaCGCCUCCUc- -3' miRNA: 3'- -CGCaGCuGAGCCugAaGCGGAGGGuc -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 11093 | 0.67 | 0.683151 |
Target: 5'- cGCGaaUCGACUUGG-CguagUCGCCcggguUCCCAc -3' miRNA: 3'- -CGC--AGCUGAGCCuGa---AGCGG-----AGGGUc -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 47982 | 0.67 | 0.672704 |
Target: 5'- cGCGUCGACUUcGACaagcaGCuCUCCUGGa -3' miRNA: 3'- -CGCAGCUGAGcCUGaag--CG-GAGGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 8950 | 0.68 | 0.651712 |
Target: 5'- aGCGuUCGAC-CGG-CUUcacCGCCUCCuCGGc -3' miRNA: 3'- -CGC-AGCUGaGCCuGAA---GCGGAGG-GUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 40147 | 0.68 | 0.624335 |
Target: 5'- gGCGUCGGCcugCGGgaucgGCUUCGCCcugacgucugcguauUCCUGGc -3' miRNA: 3'- -CGCAGCUGa--GCC-----UGAAGCGG---------------AGGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 18028 | 0.68 | 0.609602 |
Target: 5'- -aGUaCGGCUCGGGCaaCGCCUCaCGGc -3' miRNA: 3'- cgCA-GCUGAGCCUGaaGCGGAGgGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 24841 | 0.68 | 0.60855 |
Target: 5'- gGCGUCGGCggcgCGGGCggCGgccgccaaggcucCCUCaCCAGc -3' miRNA: 3'- -CGCAGCUGa---GCCUGaaGC-------------GGAG-GGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 65410 | 0.69 | 0.578175 |
Target: 5'- -aGUCGACggaGGACggUCauCCUCCCAGu -3' miRNA: 3'- cgCAGCUGag-CCUGa-AGc-GGAGGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 50850 | 0.71 | 0.467383 |
Target: 5'- gGUGgCGGCUCGGACUU-GCUcgauggucaUCCCGGg -3' miRNA: 3'- -CGCaGCUGAGCCUGAAgCGG---------AGGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 14701 | 0.72 | 0.411698 |
Target: 5'- cCGUCGACUCGaAC-UCGCCagCCAGc -3' miRNA: 3'- cGCAGCUGAGCcUGaAGCGGagGGUC- -5' |
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18256 | 5' | -56.8 | NC_004681.1 | + | 6325 | 1.1 | 0.001124 |
Target: 5'- uGCGUCGACUCGGACUUCGCCUCCCAGu -3' miRNA: 3'- -CGCAGCUGAGCCUGAAGCGGAGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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