Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18257 | 3' | -53.7 | NC_004681.1 | + | 45547 | 0.66 | 0.906732 |
Target: 5'- aCGCuGUCGUUCAUcaccacaccGAGgGCGGCcauGGAg -3' miRNA: 3'- gGCGcCAGCAAGUA---------CUUgCGCUG---CCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 52855 | 0.66 | 0.906732 |
Target: 5'- uCCGCcaGGUCGacCAUGcAGCGCGugaagacCGGGc -3' miRNA: 3'- -GGCG--CCAGCaaGUAC-UUGCGCu------GCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 13007 | 0.66 | 0.893404 |
Target: 5'- aCCGCGGaCGa-CGUGGcCGCGACuGAc -3' miRNA: 3'- -GGCGCCaGCaaGUACUuGCGCUGcCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 14465 | 0.66 | 0.886366 |
Target: 5'- uCCGCGG-CGaUCAU--GCG-GGCGGAg -3' miRNA: 3'- -GGCGCCaGCaAGUAcuUGCgCUGCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 37880 | 0.67 | 0.879084 |
Target: 5'- uUCG-GGUCGUggaaCAUGAGCGUGGCc-- -3' miRNA: 3'- -GGCgCCAGCAa---GUACUUGCGCUGccu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 45176 | 0.67 | 0.879084 |
Target: 5'- aCCGCGGcgaGggCcucGAcCGCGGCGGAu -3' miRNA: 3'- -GGCGCCag-CaaGua-CUuGCGCUGCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 35585 | 0.67 | 0.871564 |
Target: 5'- -gGUGGUCGgag--GAAgGCGACGGc -3' miRNA: 3'- ggCGCCAGCaaguaCUUgCGCUGCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 49736 | 0.67 | 0.863811 |
Target: 5'- cCCGCucccGGcacuccuucUCGUUCAccGGACGaCGACGGGa -3' miRNA: 3'- -GGCG----CC---------AGCAAGUa-CUUGC-GCUGCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 44283 | 0.67 | 0.847632 |
Target: 5'- uCCaGCGGUCGUUgGUGAuUGgGGCGc- -3' miRNA: 3'- -GG-CGCCAGCAAgUACUuGCgCUGCcu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 19588 | 0.67 | 0.83922 |
Target: 5'- cCCGCGGUCaacgagcUC-UGGGCGCGcuGCGGc -3' miRNA: 3'- -GGCGCCAGca-----AGuACUUGCGC--UGCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 51421 | 0.67 | 0.83922 |
Target: 5'- uCCGCGG-CGacgGUGAugACGuCGGCGGAc -3' miRNA: 3'- -GGCGCCaGCaagUACU--UGC-GCUGCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 10617 | 0.67 | 0.836657 |
Target: 5'- uCCGCGGUCGgacuUCGcgcccuugccgaucUGGACaaagGUGACGGu -3' miRNA: 3'- -GGCGCCAGCa---AGU--------------ACUUG----CGCUGCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 6797 | 0.68 | 0.830605 |
Target: 5'- aCCGCGGUgcucucaacccCGUcgAUGGAcCGUGACGGc -3' miRNA: 3'- -GGCGCCA-----------GCAagUACUU-GCGCUGCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 61846 | 0.68 | 0.812795 |
Target: 5'- gCCGCGGUC--UgGUGGcGCGCGAgGGc -3' miRNA: 3'- -GGCGCCAGcaAgUACU-UGCGCUgCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 62934 | 0.69 | 0.78477 |
Target: 5'- gCUGUGGUCGacuccaucuccUUCggcgGUGGugGCGGCGGc -3' miRNA: 3'- -GGCGCCAGC-----------AAG----UACUugCGCUGCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 34347 | 0.69 | 0.764341 |
Target: 5'- gCCGCGG-CGg-CGUGAggguuguGCGCGcuGCGGAa -3' miRNA: 3'- -GGCGCCaGCaaGUACU-------UGCGC--UGCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 31835 | 0.69 | 0.755408 |
Target: 5'- aUCG-GGUCGgcgccugccacUUCGgacagGAGCGCGAUGGAg -3' miRNA: 3'- -GGCgCCAGC-----------AAGUa----CUUGCGCUGCCU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 44682 | 0.7 | 0.735225 |
Target: 5'- gUGCGG-CGgcCGcGAGCGCGGCGGc -3' miRNA: 3'- gGCGCCaGCaaGUaCUUGCGCUGCCu -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 15703 | 0.7 | 0.683251 |
Target: 5'- uUCGCGGUCGUcuugUCGUcgccGAACaGUGACGaGAa -3' miRNA: 3'- -GGCGCCAGCA----AGUA----CUUG-CGCUGC-CU- -5' |
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18257 | 3' | -53.7 | NC_004681.1 | + | 24824 | 0.72 | 0.608821 |
Target: 5'- aCGCGGUCGaccUCAUcGGCGuCGGCGGc -3' miRNA: 3'- gGCGCCAGCa--AGUAcUUGC-GCUGCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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