Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18257 | 3' | -53.7 | NC_004681.1 | + | 37880 | 0.67 | 0.879084 |
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Target: 5'- uUCG-GGUCGUggaaCAUGAGCGUGGCc-- -3' miRNA: 3'- -GGCgCCAGCAa---GUACUUGCGCUGccu -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 45176 | 0.67 | 0.879084 |
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Target: 5'- aCCGCGGcgaGggCcucGAcCGCGGCGGAu -3' miRNA: 3'- -GGCGCCag-CaaGua-CUuGCGCUGCCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 14465 | 0.66 | 0.886366 |
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Target: 5'- uCCGCGG-CGaUCAU--GCG-GGCGGAg -3' miRNA: 3'- -GGCGCCaGCaAGUAcuUGCgCUGCCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 13007 | 0.66 | 0.893404 |
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Target: 5'- aCCGCGGaCGa-CGUGGcCGCGACuGAc -3' miRNA: 3'- -GGCGCCaGCaaGUACUuGCGCUGcCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 52855 | 0.66 | 0.906732 |
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Target: 5'- uCCGCcaGGUCGacCAUGcAGCGCGugaagacCGGGc -3' miRNA: 3'- -GGCG--CCAGCaaGUAC-UUGCGCu------GCCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 45547 | 0.66 | 0.906732 |
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Target: 5'- aCGCuGUCGUUCAUcaccacaccGAGgGCGGCcauGGAg -3' miRNA: 3'- gGCGcCAGCAAGUA---------CUUgCGCUG---CCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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