Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18257 | 5' | -60.6 | NC_004681.1 | + | 12462 | 0.66 | 0.569198 |
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Target: 5'- --gGCCCCgg--CCACGAGcGGUUCGu -3' miRNA: 3'- acaCGGGGagcaGGUGCUC-CCAGGCu -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 45600 | 0.66 | 0.538864 |
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Target: 5'- gGUGCCuCCUccgCGUCgGCcaAGGGUaCCGAg -3' miRNA: 3'- aCACGG-GGA---GCAGgUGc-UCCCA-GGCU- -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 634 | 0.69 | 0.370597 |
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Target: 5'- uUGUGCCCgUCGUugagggacucgcggCCGCGGaagauggccGGGUCCa- -3' miRNA: 3'- -ACACGGGgAGCA--------------GGUGCU---------CCCAGGcu -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 54006 | 0.69 | 0.356863 |
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Target: 5'- --aGCCgggggUCUCGcauugcuggcuUCCACGAGGGUCCGc -3' miRNA: 3'- acaCGG-----GGAGC-----------AGGUGCUCCCAGGCu -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 10572 | 0.69 | 0.356863 |
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Target: 5'- gGUGUCCUcaccgaugCGuUCCAUGGGGGUCCu- -3' miRNA: 3'- aCACGGGGa-------GC-AGGUGCUCCCAGGcu -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 62551 | 0.71 | 0.283457 |
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Target: 5'- cGUGCCCCUCGgCCAUucGGGUuucCUGGg -3' miRNA: 3'- aCACGGGGAGCaGGUGcuCCCA---GGCU- -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 30940 | 0.72 | 0.251473 |
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Target: 5'- cGUG-CCgUCcUCCACGAuGGGUCCGGg -3' miRNA: 3'- aCACgGGgAGcAGGUGCU-CCCAGGCU- -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 52453 | 0.74 | 0.18213 |
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Target: 5'- --cGCUUgCUCGcCCACGAGGGUCCGu -3' miRNA: 3'- acaCGGG-GAGCaGGUGCUCCCAGGCu -5' |
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| 18257 | 5' | -60.6 | NC_004681.1 | + | 7106 | 1.09 | 0.000552 |
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Target: 5'- aUGUGCCCCUCGUCCACGAGGGUCCGAa -3' miRNA: 3'- -ACACGGGGAGCAGGUGCUCCCAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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