Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18258 | 3' | -55.3 | NC_004681.1 | + | 30839 | 0.66 | 0.854184 |
Target: 5'- cCCggacCCAUCGUGgaGGACGGCAcgccgCCCGc -3' miRNA: 3'- -GGa---GGUGGCACa-UCUGCUGUca---GGGC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 7441 | 0.66 | 0.829034 |
Target: 5'- gCUUCGCCGcGgcGGCGGgguCAGcCCCGg -3' miRNA: 3'- gGAGGUGGCaCauCUGCU---GUCaGGGC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 57089 | 0.66 | 0.811297 |
Target: 5'- gCCUCCGCCaggacggacuaGUGccGACGAUcuugaaggccaAGUCCCc -3' miRNA: 3'- -GGAGGUGG-----------CACauCUGCUG-----------UCAGGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 22271 | 0.67 | 0.764083 |
Target: 5'- aCUCCG-CGUGgcGGCGugGGcgCCCu -3' miRNA: 3'- gGAGGUgGCACauCUGCugUCa-GGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 56130 | 0.67 | 0.754225 |
Target: 5'- gUCUCCACgGUGaAGGCGAUgcggaGGUCgCCc -3' miRNA: 3'- -GGAGGUGgCACaUCUGCUG-----UCAG-GGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 38928 | 0.68 | 0.734175 |
Target: 5'- cCCUCCacgcuGCCGccGUAGGCccgGAUGGUCUCGg -3' miRNA: 3'- -GGAGG-----UGGCa-CAUCUG---CUGUCAGGGC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 47021 | 0.68 | 0.724003 |
Target: 5'- gCCU-CACCGUagGUAGugccaGCGAacCAGUCCCGc -3' miRNA: 3'- -GGAgGUGGCA--CAUC-----UGCU--GUCAGGGC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 75630 | 0.68 | 0.713746 |
Target: 5'- gCUCUACCGUGUcGugGAUcGUUCgGg -3' miRNA: 3'- gGAGGUGGCACAuCugCUGuCAGGgC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 1784 | 0.68 | 0.703415 |
Target: 5'- cCCUCCucACCGUcGUAGGCcaGGCAGagcgaguacaUCCCa -3' miRNA: 3'- -GGAGG--UGGCA-CAUCUG--CUGUC----------AGGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 3556 | 0.69 | 0.69302 |
Target: 5'- gUCUCCACgGUGgGGACGGgcGUCaCCGa -3' miRNA: 3'- -GGAGGUGgCACaUCUGCUguCAG-GGC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 396 | 0.69 | 0.661548 |
Target: 5'- gCUUCCGCgGgucgGU-GAUGACAGUUCCa -3' miRNA: 3'- -GGAGGUGgCa---CAuCUGCUGUCAGGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 59319 | 0.69 | 0.640424 |
Target: 5'- aCUCCugCGUGgccaggaccccGGACGGCGGagCCCu -3' miRNA: 3'- gGAGGugGCACa----------UCUGCUGUCa-GGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 31663 | 0.71 | 0.577157 |
Target: 5'- gCUUCugCGUcugcucucgGUAGACG-CGGUCCCa -3' miRNA: 3'- gGAGGugGCA---------CAUCUGCuGUCAGGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 56039 | 0.73 | 0.466002 |
Target: 5'- gCCUUCACCGUGgAGACG-CAGcugUCCGu -3' miRNA: 3'- -GGAGGUGGCACaUCUGCuGUCa--GGGC- -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 2945 | 0.75 | 0.358724 |
Target: 5'- gCCUCgGggacggugaCCGUGUAG-CGGCGGUCCCc -3' miRNA: 3'- -GGAGgU---------GGCACAUCuGCUGUCAGGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 27196 | 0.78 | 0.226852 |
Target: 5'- gCCUCCACCGgGUgcgAGugGACGGUgCCa -3' miRNA: 3'- -GGAGGUGGCaCA---UCugCUGUCAgGGc -5' |
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18258 | 3' | -55.3 | NC_004681.1 | + | 7652 | 1.11 | 0.001333 |
Target: 5'- uCCUCCACCGUGUAGACGACAGUCCCGg -3' miRNA: 3'- -GGAGGUGGCACAUCUGCUGUCAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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