Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18259 | 5' | -58.1 | NC_004681.1 | + | 7139 | 0.66 | 0.695131 |
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Target: 5'- cGGUCgUUCAUGAacgcgacGGAGACGGUgCUGa -3' miRNA: 3'- -CCAGgGGGUACUc------CCUCUGCCA-GACc -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 59093 | 0.66 | 0.680745 |
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Target: 5'- -cUCCCCCAcGAGGucuucgagcaccuGGGCGGcCUGGu -3' miRNA: 3'- ccAGGGGGUaCUCCc------------UCUGCCaGACC- -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 18921 | 0.66 | 0.671452 |
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Target: 5'- --gCCCCCAUGAGGcugacuucacccucGGGAUGGg-UGGu -3' miRNA: 3'- ccaGGGGGUACUCC--------------CUCUGCCagACC- -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 27459 | 0.67 | 0.621596 |
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Target: 5'- gGGUUCCgCCGuUGAGGGucagcucgaauggGGGCGGUgUGa -3' miRNA: 3'- -CCAGGG-GGU-ACUCCC-------------UCUGCCAgACc -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 66024 | 0.68 | 0.550459 |
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Target: 5'- aGGUCagugCCaCCGUGAGGGAccuuGCGGUCgcccaGGa -3' miRNA: 3'- -CCAG----GG-GGUACUCCCUc---UGCCAGa----CC- -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 46478 | 0.69 | 0.49072 |
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Target: 5'- aGGUCUCCCccGAGGaGGACGa-CUGGa -3' miRNA: 3'- -CCAGGGGGuaCUCCcUCUGCcaGACC- -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 3464 | 0.71 | 0.416169 |
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Target: 5'- cGUCCCCaccgu-GGAGACGGUCUGc -3' miRNA: 3'- cCAGGGGguacucCCUCUGCCAGACc -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 46701 | 0.71 | 0.407342 |
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Target: 5'- gGGUCCUCgGcGAGuGGGcCGGUCUGGg -3' miRNA: 3'- -CCAGGGGgUaCUCcCUCuGCCAGACC- -5' |
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| 18259 | 5' | -58.1 | NC_004681.1 | + | 7730 | 1.11 | 0.000719 |
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Target: 5'- gGGUCCCCCAUGAGGGAGACGGUCUGGc -3' miRNA: 3'- -CCAGGGGGUACUCCCUCUGCCAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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