Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18260 | 3' | -55.3 | NC_004681.1 | + | 57818 | 0.66 | 0.817339 |
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Target: 5'- -aGGugGCGAAGGagcgGGGCuggCccCUCCc -3' miRNA: 3'- gaCCugCGCUUCCg---CUCGua-Ga-GAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 58180 | 0.67 | 0.728521 |
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Target: 5'- -cGGAUGCccGGuaucGGGCGAGUGUCUCagguucgUCCu -3' miRNA: 3'- gaCCUGCG--CU----UCCGCUCGUAGAG-------AGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 60126 | 0.66 | 0.77974 |
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Target: 5'- -gGGAgcCGcCGAAGGCGAGCAggugC-CCa -3' miRNA: 3'- gaCCU--GC-GCUUCCGCUCGUaga-GaGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 61835 | 0.67 | 0.748968 |
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Target: 5'- gUGGcGCGCGAGGGCGGccuccuuGCGgacggaCUCCa -3' miRNA: 3'- gACC-UGCGCUUCCGCU-------CGUaga---GAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 62714 | 0.7 | 0.556458 |
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Target: 5'- -cGGACgggGUGggGGCGuagaugaccuccaaAGCAUCcCUCCg -3' miRNA: 3'- gaCCUG---CGCuuCCGC--------------UCGUAGaGAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 62962 | 0.77 | 0.249523 |
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Target: 5'- gUGGugGCGGcggcGGcGCGAGCAgCUUUCCg -3' miRNA: 3'- gACCugCGCU----UC-CGCUCGUaGAGAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 63578 | 0.68 | 0.698245 |
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Target: 5'- gUGGGCGgGGA-GUGAGUAUCUUcCCc -3' miRNA: 3'- gACCUGCgCUUcCGCUCGUAGAGaGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 70145 | 0.66 | 0.808192 |
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Target: 5'- gCUGGAgaCGCuGAGGGCuGAGCuccacgCUgagCUCCg -3' miRNA: 3'- -GACCU--GCG-CUUCCG-CUCGua----GA---GAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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