Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18261 | 3' | -57.8 | NC_004681.1 | + | 9237 | 0.66 | 0.719925 |
Target: 5'- -cGCGCGACuGggagcUCCUGcGCCuGACGAa -3' miRNA: 3'- gcUGCGCUGuCac---AGGAC-CGG-CUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 9588 | 0.66 | 0.709745 |
Target: 5'- uCGGCGCGcACGGUGaCCUuGCCaucaACGAa -3' miRNA: 3'- -GCUGCGC-UGUCACaGGAcCGGc---UGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 5297 | 0.66 | 0.699496 |
Target: 5'- uGugGCGACAGcucggCCaUGGCgGGCGc -3' miRNA: 3'- gCugCGCUGUCaca--GG-ACCGgCUGCu -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 10585 | 0.66 | 0.689187 |
Target: 5'- gGACaaagGUGACGGUGUCCUcaCCGAUGc -3' miRNA: 3'- gCUG----CGCUGUCACAGGAccGGCUGCu -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 51555 | 0.66 | 0.689187 |
Target: 5'- uGAgGCGACGcUG-CCUGGCUuuguccuuGACGAu -3' miRNA: 3'- gCUgCGCUGUcACaGGACCGG--------CUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 1285 | 0.66 | 0.678829 |
Target: 5'- gGGCGCc-CAGgaaGUCugggacgagCUGGCCGACGAc -3' miRNA: 3'- gCUGCGcuGUCa--CAG---------GACCGGCUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 16529 | 0.66 | 0.678829 |
Target: 5'- gGugGCGGCAacgGUCCccacggcGGCCGugGu -3' miRNA: 3'- gCugCGCUGUca-CAGGa------CCGGCugCu -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 11357 | 0.66 | 0.668431 |
Target: 5'- gGACGuUGAUgaGGcUGgcgaCCUGGCUGACGAa -3' miRNA: 3'- gCUGC-GCUG--UC-ACa---GGACCGGCUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 49579 | 0.66 | 0.668431 |
Target: 5'- cCGGCGacugGGCAGUagggcuugccGUCCUuguagcgggGGCCGugGAa -3' miRNA: 3'- -GCUGCg---CUGUCA----------CAGGA---------CCGGCugCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 65602 | 0.67 | 0.647551 |
Target: 5'- uCGAUGuCGAUAGUGaggaUUUUGGCCGucACGAc -3' miRNA: 3'- -GCUGC-GCUGUCAC----AGGACCGGC--UGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 46429 | 0.67 | 0.637088 |
Target: 5'- uGAUGCccagcuuccACAGUG-CCUGGCCcACGAu -3' miRNA: 3'- gCUGCGc--------UGUCACaGGACCGGcUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 61879 | 0.68 | 0.584875 |
Target: 5'- uGAUugGCGACguAGaGcUCCUGGCCGAUGGc -3' miRNA: 3'- gCUG--CGCUG--UCaC-AGGACCGGCUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 16743 | 0.68 | 0.564186 |
Target: 5'- aGACccuCGAgGGUGUCCgGGCUGAUGc -3' miRNA: 3'- gCUGc--GCUgUCACAGGaCCGGCUGCu -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 30158 | 0.68 | 0.553913 |
Target: 5'- -uGCGCGACAGcgGgagcUCCgaGGCCGAUGGc -3' miRNA: 3'- gcUGCGCUGUCa-C----AGGa-CCGGCUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 18220 | 0.68 | 0.553913 |
Target: 5'- cCGACcUGACccGGUG-CCUGGCCGGgGGc -3' miRNA: 3'- -GCUGcGCUG--UCACaGGACCGGCUgCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 21291 | 0.69 | 0.493659 |
Target: 5'- aGugGCGACGcUGUCCauucGGCCGuuaauCGAa -3' miRNA: 3'- gCugCGCUGUcACAGGa---CCGGCu----GCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 52304 | 0.7 | 0.483899 |
Target: 5'- uGGCGgGGCcGU-UCUUGGCCGugGAu -3' miRNA: 3'- gCUGCgCUGuCAcAGGACCGGCugCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 6521 | 0.77 | 0.176828 |
Target: 5'- aGACGCGACAGgaGUgCUGGCCGGuCGc -3' miRNA: 3'- gCUGCGCUGUCa-CAgGACCGGCU-GCu -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 52272 | 0.78 | 0.159414 |
Target: 5'- gCGACGCgGACGuUGUCCUccuccuccagaaGGCCGACGAc -3' miRNA: 3'- -GCUGCG-CUGUcACAGGA------------CCGGCUGCU- -5' |
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18261 | 3' | -57.8 | NC_004681.1 | + | 9155 | 1.09 | 0.001035 |
Target: 5'- gCGACGCGACAGUGUCCUGGCCGACGAa -3' miRNA: 3'- -GCUGCGCUGUCACAGGACCGGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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